Studies of the mechanisms of radiosensitization of bacterial and mammalian cells by Diamide.
Studies of the mechanisms of radiosensitization of bacterial and mammalian cells by Diamide.
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二酰胺对细菌和哺乳动物细胞放射增敏机制的研究。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
G. Adams
中科院分区：
文献类型：
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作者：
M. Watts;D. Whillans;G. Adams
二胺至少通过两种机制使细菌和哺乳动物细胞对辐射敏感。V79-GL1中国仓鼠细胞的致敏作用主要是由于生存曲线肩部的减少，在缺氧和缺氧条件下均有观察到，并且与一些硝基咪唑对缺氧细胞的致敏作用是附加的。相比之下，辐射耐药生物的致敏作用，sodonmicrococcus，它有明显的肩部，完全是剂量修饰的。在使用粘质沙雷氏菌的快速混合研究中，已经确定了两种致敏机制。辐照前与Daimide接触4 ~ 40 msec后，增强比稳定在1.6；接触10min后为2.5。对于V79-GL1细胞，短时间内不发生致敏；同样，在辐照后4毫秒将二胺添加到细菌或哺乳动物细胞中也可见非二胺。结果表明，二胺的敏化机制至少有两种，其中一种类似于电子亲合型的敏化机制。
Diamide sensitizes bacterial and mammalian cells to radiation by at least two mechanisms. Sensitization of V79-GL1 Chinese Hamster cells is due mainly to a reduction of a reduction of the survival-curve shoulder, is observed both in oxygen and in hypoxia,and is additive to the sensitization of hypoxic cells by some nitroimidazoles. In contrast, sensitization of the radioresistant organism, Micrococcus sodonensis, which has apronounced shoulder, is entirely dose-modifying. In a rapid-mix study using Serratia marcescens, two mechanisms of sensitization have been identified. After 4 to 40 msec preirradiation contact with Daimide, the enhancement ratio is constant at 1.6; after 10min contact, it is 2.5. With V79-GL1 cells, no sensitization occurs in the short-time-range; likewise non is seen when Diamide is added to bacteria or mammalian cells 4 msec after irradiation. It is concluded that Diamide sensitizes by at least two mechanisms, one of which resembles that of the electron-affinic type.