MiR-455 enhances adipogenic differentiation of 3T3-L1 cells through targeting uncoupling protein-1(Retracted article. See vol. 72, pg. 192, 2017)
MiR-455 enhances adipogenic differentiation of 3T3-L1 cells through targeting uncoupling protein-1(Retracted article. See vol. 72, pg. 192, 2017)
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DOI：
10.1691/ph.2016.6734
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发表时间：
2016-11-01
期刊：
影响因子：
1.6
通讯作者：
Guo, Xirong
中科院分区：
文献类型：
--
作者：
Cai, Zhiyong;Liu, Jindi;Guo, Xirong
越来越多的证据表明，microRNAs （miRNAs）在调节脂肪组织中控制脂肪形成、胰岛素抵抗和炎症等过程的途径中起着重要作用。前脂肪细胞的成脂分化是一个复杂的过程，受多种因素调控，包括mirna和细胞因子。MiR-455是一种众所周知的促进脂肪形成的miRNA。解偶联蛋白1 （Uncoupling protein-1, UCP-1）是一种肝素结合生长因子，在脂肪形成中起负作用。在这项研究中，我们证明UCP-1是miR-455在3T3-L1前脂肪细胞成脂分化过程中的靶基因。MiR-455通过与小鼠UCP-1编码区的MiR-455靶点相互作用下调UCP-1的表达。靶位上游的罕见密码子调控mir -455诱导的UCP-1的翻译敲低，这为脂肪分化的机制提供了更多的见解。因此，这些结果表明，miR-455在3T3-L1细胞中的加速成脂作用至少部分是由于抑制UCP-1。
Accumulating evidence suggests that microRNAs (miRNAs) play an important role in regulating the pathways in adipose tissue that control processes such as adipogenesis, insulin resistance, and inflammation. Adipogenic differentiation of preadipocytes is a complex process regulated by various factors including miRNAs and cytokines. MiR-455 is a well-known miRNA that enhances adipogenesis. Uncoupling protein-1 (UCP-1), a heparin binding growth factor, plays a negative role in adipogenesis. In this investigation, we demonstrate that UCP-1 is a target gene of miR-455 during adipogenic differentiation in 3T3-L1 preadipocytes. MiR-455 downregulates UCP-1 expression through interaction with a target site of miR-455 in the coding region of mouse UCP-1. The rare codons upstream of the target site regulate miR-455-induced translational knockdown of UCP-1, which provides more insight into the mechanism of adipogenic differentiation. Thus, these results suggest that the acceerative adipogenic effect of miR-455 in 3T3-L1 cells is due, at least in part, to suppression of UCP-1.