Effects of Fe3+ on Acute Toxicity and Regeneration of Planarian (Dugesia japonica) at Different Temperatures
Effects of Fe3+ on Acute Toxicity and Regeneration of Planarian (Dugesia japonica) at Different Temperatures
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DOI：
10.1155/2019/8591631
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发表时间：
2019-08-22
影响因子：
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通讯作者：
Xu, Zhenbiao
中科院分区：
文献类型：
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作者：
Ding, Xue;Song, Linxia;Xu, Zhenbiao
Objective.研究不同温度下不同浓度的Fe 3+对真涡虫的急性毒性及再生的影响。法用40 mg/l、50 mg/l、60 mg/l和70 mg/l的Fe 3+溶液处理真涡虫，并分别置于15 ℃、20 ℃和25 ℃下，观察真涡虫的死亡率和中毒模式。此外，将真涡虫切成头、躯干和尾三部分，然后置于浓度为10 mg/l、15 mg/l、20 mg/l和30 mg/l的Fe 3+溶液中，并分别置于15 ℃、20 ℃和25 ℃下，研究真涡虫的再生率。结果在同一温度下，Fe ~（3+）浓度在40 ~ 70 mg/l范围内，随着Fe ~（3+）浓度的增加，真涡虫的死亡率增加;在相同浓度、不同温度下，20 ℃时，真涡虫死亡速度最快，25 ℃次之，15 ℃最低，表明在20 ℃的合适温度下，Fe 3+的毒性作用可以被加速。在同一温度下，在低浓度Fe ~（3+）（10 ~ 30 mg/l）下，随着Fe ~（3+）浓度的增加，真涡虫的再生率逐渐降低;在相同浓度和不同温度下，20 ℃和25 ℃下的真涡虫再生速度较快，但20 ℃和25 ℃之间的差异较小，而在15 ℃时最慢，表明低温显著影响真涡虫的再生速度。研究还发现，真涡虫头部、躯干和尾部的再生速度不同，头部再生最快，躯干其次，尾部最慢。结论Fe 3+对真涡虫的存活和再生有明显的毒性作用;真涡虫对Fe 3+敏感，可用于检测Fe 3+对水体的污染;另外，温度可影响Fe 3+的毒性作用，从而影响真涡虫的存活和再生。因此，在检测水体Fe 3+污染时应考虑温度的影响。
Objective. To investigate the effects of different concentrations of Fe3+ on the acute toxicity and regeneration of planarian at different temperatures. Method. The planarians were treated with 40 mg/l, 50 mg/l, 60 mg/l, and 70 mg/l Fe3+ solution and placed in 15 degrees C, 20 degrees C, and 25 degrees C, respectively, to observe the mortality and the poisoning pattern of the planarian. In addition, the planarians were cut into three parts of head, trunk, and tail, then placed in Fe3+ solution at concentrations of 10 mg/l, 15 mg/l, 20 mg/l, and 30 mg/l, and placed in 15 degrees C, 20 degrees C, and 25 degrees C respectively, and the regeneration rate of the planarian was investigated. Results. At the same temperature, in the concentration of Fe3+ from 40 mg/l to 70 mg/l, the mortality of the planarian increased with the increasing of the concentration of Fe3+; at the same concentration and different temperatures, the death speed of the planarian is the fastest at 20 degrees C, the next at 25 degrees C, and the lowest at 15 degrees C, indicating that the toxic effect of Fe3+ can be accelerated at a suitable temperature of 20 degrees C. At the same temperature, in the low concentration of Fe3+ from 10 mg/l to 30 mg/l, the regeneration rate of the planarian gradually decreased with the increasing of the concentration of Fe3+; at the same concentration and different temperature, the regeneration rate of planarian was faster at 20 degrees C and 25 degrees C, but the difference between 20 degrees C and 25 degrees C was small, and the slowest at 15 degrees C, indicating that the low temperature significantly affects the planarian regeneration speed. The study also found the regeneration rates of the head, trunk, and tail of the planarian were different; the head regeneration was the fastest, the trunk was the second, and the tail was the slowest. Conclusion. Fe3+ had obvious toxic effects on the survival and regeneration of planarian; the planarian is sensitive to Fe3+ and may be used to detect Fe3+ water pollution; in addition, temperature can affect the toxic effects of Fe3+ and thus affect the survival and regeneration of the planarian. Therefore, the temperature should be taken into consideration when detecting water Fe3+ pollution.