MOLECULAR-WEIGHT AND NET CHARGE OF PEROXIDASE ISOZYMES IN F1 HYBRIDS BETWEEN L AND S FLAX GENOTROPHS
MOLECULAR-WEIGHT AND NET CHARGE OF PEROXIDASE ISOZYMES IN F1 HYBRIDS BETWEEN L AND S FLAX GENOTROPHS
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DOI：
10.1007/bf00484069
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发表时间：
1982-01-01
影响因子：
2.4
通讯作者：
FIELDES, MA
中科院分区：
文献类型：
--
作者：
TYSON, H;FIELDES, MA
Durrant's L和Durrant's S亚麻基因营养型杂种F1的阴离子过氧化物酶同工酶的相对迁移率与L亲本基本相同。在一系列丙烯酰胺凝胶浓度下比较了双亲及其F1的同工酶，并绘制了相对迁移率对凝胶浓度的对数图。还检查了相对于内标Hb的相对迁移率与浓度的关系图。这两种方法都提供了证据表明，明显的MW修饰导致了双亲之间的迁移率的变化，并且F1与L的相似性，双亲是显性等位基因纯合的，控制了至少2种同工酶的迁移率变化。
In the F1 hybrids between Durrant''s L and Durrant''s S flax genotrophs, the relative mobilities of the anionic peroxidase isozymes were essentially the same as those in the L parent. The isozymes in both parents and their F1 were compared over a range of acrylamide gel concentrations, with plots of log relative mobility against gel concentration. Plots of comparative mobility, relative to the internal standard Hb, against concentration were also examined. Both approaches provided evidence that apparent MW modifications underlay the shift in mobility between the parents and the resemblance of the F1 to L, the parent which was homozygous for the dominant alleles controlling the mobility shift for at least 2 of the isozymes.