EFFECT OF AGE ON PYRUVATE-KINASE AND LACTATE-DEHYDROGENASE DISTRIBUTION IN RAT-KIDNEY
EFFECT OF AGE ON PYRUVATE-KINASE AND LACTATE-DEHYDROGENASE DISTRIBUTION IN RAT-KIDNEY
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DOI：
10.1152/ajplegacy.1974.226.5.1227
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发表时间：
1974-01-01
影响因子：
--
通讯作者：
FAGIOLI, S
中科院分区：
文献类型：
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作者：
BURCH, HB;LOWRY, OH;FAGIOLI, S
海伦·伯奇B奥利弗·H洛瑞，佩里，露西范，和苏珊法吉利。年龄对大鼠肾脏丙酮酸激酶和乳酸脱氢酶分布的影响。Am. J. Physiol.226（s）：1227-1231. 1974.~丙酮酸激酶和乳酸脱氢酶测定在一些或所有的以下结构的大鼠肾脏从胎龄后期到成人：肾小球，近曲和远曲和直小管，薄肢区的髓质，新生带，间充质，和髓射线。将重量为0.01- 1 pg的样品解剖用于分析。丙酮酸激酶在成人肾脏中的范围为MO倍，从近曲小管（最低）、肾小球和近端直小管（中间）、远端小管（高）到薄肢区（最高）。乳酸脱氢酶与丙酮酸激酶的比例从近曲小管的15到细肢区的0.3不等。在所有年龄的丙酮酸激酶的活性在低浓度的P-丙酮酸刺激果糖二磷酸在近端小管，但不是在其他结构。在胚胎发育过程中，糖酵解是一种浪费。糖异生可能仅限于近端小管。因此，丙酮酸激酶不仅在近端小管中含量低，而且易受变构控制，这似乎很重要。肾小管;肾单位发育;别构丙酮酸激酶
BURCH, HELEN B., OLIVER H. LOWRY, SG PERRY, LUCY FAN, AND SUSAN FAGIOLI. E $ ect of age on pyruvate kinase and lactate dehydrogenase distribution in rat kidney. Am. J. Physiol. 226 (s): 1227-1231. 1974.~ Pyruvate kinase and lactate dehydrogenase were measured in some or all of the following structures of rat kidney from late fetal age to the adult: glomeruli, proximal, and distal convoluted and straight tubules, thin-limb areas of the medulla, the neogenic zone, mesenchyme, and medullary ray. Samples weighing 0.01-l pg were dissected for analysis. Pyruvate kinase ranged MO-fold in the adult kidney from the proximal convoluted tubules (lowest), the glomeruli and proximal straight tubules (intermediate), the distal tubules (high), to the thin-limb area (highest). The ratio of lactate dehydrogenase to pyruvate kinase varied from 15 in proximal convoluted tubules to 0.3 in thin-limb areas. At all ages the activity of pyruvate kinase at low P-pyruvate concentration was stimulated by fructose diphosphate in proximal tubules but not in other structures. During gluconeogenesis glycolysis would be wasteful. Gluconeogenesis is probably restricted to the proximal tubules. It therefore seems significant that pyruvate kinase is not only low in proximal tubules, but also susceptible to allosteric control. renal tubules; development of nephrons; allosteric pyruvate kinase