Identification of insulin-like growth factor-binding protein-3 (IGFBP-3) and IGFBP-2 in human follicular fluid.
Identification of insulin-like growth factor-binding protein-3 (IGFBP-3) and IGFBP-2 in human follicular fluid.
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DOI：
10.1210/jcem-71-5-1330
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发表时间：
1990-11
期刊：
影响因子：
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通讯作者：
L. C. Giudice;Eleanor M. Farrell;H. Pham;R. G. Rosenfeld
中科院分区：
文献类型：
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作者：
L. C. Giudice;Eleanor M. Farrell;H. Pham;R. G. Rosenfeld
两种主要的胰岛素样生长因子结合蛋白，IGFBP-2和IGFBP-3，通过Western配体印迹法从人绝经期促性腺激素（LH和FSH）刺激卵巢期间从黄体化卵泡抽吸的人卵泡液（FF）中鉴定。IGFBP-1也存在于FF中，并且存在于血清中的分子量为24000道尔顿的IGFBP似乎不存在于FF中。使用IGFBP特异性抗血清确认IGFBP-1、IGFBP-2和IGFBP-3的鉴定。在卵母细胞收获时测量患者血清和FF中的IGF-I和IGF-II水平，并且两种肽的血清水平始终高于FF水平。人FF中三种主要血清IGFBPs的发现表明，卵巢中的IGF调节可能受到这组调节BP的复杂控制。
Two major insulin-like growth factor-binding proteins, IGFBP-2 and IGFBP-3, were identified by Western ligand blotting of human follicular fluid (FF) aspirated from luteinizing follicles during ovarian stimulation with human menopausal gonadotropin (LH and FSH). IGFBP-1 was also present in FF, and an IGFBP with a mol wt of 24000 daltons, present in serum, appeared to be absent in FF. Identification of IGFBP-1, IGFBP-2, and IGFBP-3 was confirmed using IGFBP-specific antisera. IGF-I and IGF-II levels were measured in patient serum and FF at the time of oocyte harvest, and serum levels of both peptides were consistently higher than FF levels. The finding of three major serum IGFBPs in human FF suggests that IGF regulation in the ovary is probably under complex control by this set of regulatory BPs.