Nitric oxide radical scavenging active components from Phyllanthus emblica L.
Nitric oxide radical scavenging active components from Phyllanthus emblica L.
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DOI：
10.1007/s11130-006-0001-0
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发表时间：
2006-03-01
影响因子：
4
通讯作者：
Karunakaran, R. Joel
中科院分区：
文献类型：
--
作者：
Kumaran, A.;Karunakaran, R. Joel
采用活性导向的分离纯化方法对余甘子中清除一氧化氮（NO）的活性成分进行了鉴定。余甘子的干燥果皮用甲醇提取，然后分离成己烷、乙酸乙酯和水馏分。其中，只有乙酸乙酯相显示出较强的NO清除活性在体外，与水和正己烷相相比。然后使用Sephadex LH-20色谱法对乙酸乙酯级分进行分离和纯化。通过光谱方法（H-1 NMR、C-13 NMR和MS）和与文献值的比较，鉴定了五种显示出强NO清除活性的化合物，它们是没食子酸、没食子酸甲酯、柯里拉京、呋辛和老鹳草素。此外，还对分离的化合物进行了HPLC鉴定和定量。没食子酸被认为是乙酸乙酯提取物中的主要化合物和老鹳草素表现出最高的NO清除活性之间的分离的化合物。
An activity-directed fractionation and purification process was used to identify the nitric oxide (NO) scavenging components of Phyllanthus emblica. Dried fruit rind of P. emblica was extracted with methanol and then separated into hexane, ethyl acetate, and water fractions. Among these only the ethyl acetate phase showed strong NO scavenging activity in vitro, when compared with water and hexane phases. The ethyl acetate fraction was then subjected to separation and purification using Sephadex LH-20 chromatography. Five compounds showing strong NO scavenging activity were identified by spectral methods (H-1 NMR, C-13 NMR, and MS) and by comparison with literature values to be Gallic acid, Methyl gallate, Corilagin, Furosin, and Geraniin. In addition, HPLC identification and quantification of isolated compounds were also performed. Gallic acid was found to be a major compound in the ethyl acetate extract and Geraniin showed highest NO scavenging activity among the isolated compounds.