MOLECULAR-CLONING OF THE 6 MESSENGER-RNA SPECIES OF INFECTIOUS HEMATOPOIETIC NECROSIS VIRUS, A FISH RHABDOVIRUS, AND GENE ORDER DETERMINATION BY R-LOOP MAPPING
MOLECULAR-CLONING OF THE 6 MESSENGER-RNA SPECIES OF INFECTIOUS HEMATOPOIETIC NECROSIS VIRUS, A FISH RHABDOVIRUS, AND GENE ORDER DETERMINATION BY R-LOOP MAPPING
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DOI：
10.1128/jvi.53.2.469-476.1985
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发表时间：
1985-01-01
影响因子：
5.4
通讯作者：
LEONG, JC
中科院分区：
文献类型：
--
作者：
KURATH, G;AHERN, KG;LEONG, JC
构建了携带传染性血细胞坏死病毒基因片段c[互补]DNA序列的重组表达载体，并将其克隆到大肠杆菌中。21个克隆的质粒的鉴定通过与mRNA杂交，确定了与6个病毒mRNA物种中的每一个都具有同源性的质粒组。R环定位结果表明，感染性造血坏死病毒基因组上的基因序列为(3‘’)N-M1-M2-G-NV-L(5‘’)。
Plasmids carrying c[complementary]DNA sequences to the mRNA species of infectious hematopoietic necrosis virus were constructed and cloned into Escherichia coli. Characterization of 21 cloned plasmids by hybridization to mRNA blots identified sets of plasmids with homology to each of the 6 viral mRNA species. R-loop mapping with these cDNA plasmids determined that the gene order on the infectious hematopoietic necrosis virus genome is (3'')N-M1-M2-G-NV-L(5'').