Plasma metabolomic profiling of repeated restraint stress in rats
Plasma metabolomic profiling of repeated restraint stress in rats
复制标题
大鼠重复束缚应激的血浆代谢组学分析
DOI：
10.1016/j.jchromb.2020.122294
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发表时间：
2020-12-01
影响因子：
3
通讯作者：
Wei, Shanshan
中科院分区：
文献类型：
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作者：
Chen, Shuai;Lu, Dengcheng;Wei, Shanshan
暴露于反复的压力与许多流行疾病的发生和发展有关。在这里，我们评估了一个重复的约束压力（RRS）的范例对雄性Wistar大鼠的影响，并比较了全球血浆代谢产物在应激和非应激大鼠使用超高效液相色谱-四极杆飞行时间质谱（UHPLC/Q-TOF-MS）的方法和多变量统计分析。数据显示，10天的RRS诱导体重减轻和焦虑样行为，可能是通过升高大鼠的血浆皮质酮水平。两组的代谢特征不同。鉴定了17种差异代谢物，并认为它们是应激过程中的潜在生物标志物，包括组氨酸代谢、神经递质合成和胆汁酸代谢。此外，在同一模型中检查了L-肌肽（一种已鉴定的代谢物），发现其可减轻RRS大鼠的体重减轻和焦虑样行为。因此，这些研究结果描述了重复应激的代谢组学特征，并为应激诱导的偏转的病理生理机制和潜在的药理学干预提供了新的见解。
Exposure to repeated stress is associated with the occurrence and development of many prevalent diseases. Here, we evaluated the impact of a repeated restraint stress (RRS) paradigm on male Wistar rats and compared global plasma metabolites in stressed and unstressed rats using an ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) approach and multivariate statistical analysis. The data showed that ten days of RRS induced weight loss and anxiety-like behavior, possibly through elevating plasma corticosterone levels in rats. Different metabolic characteristics were found between the two groups. Seventeen differential metabolites were identified and considered as potential biomarkers in the stress process, including histidine metabolism, neurotransmitter synthesis, and bile acid metabolism. Furthermore, L-carnosine, an identified metabolite, was examined in the same model and found to attenuate weight loss and anxiety-like behavior in RRS rats. Hence, these findings describe the metabolomic profiling of repeated stress and provide new insights into the pathophysiological mechanism of stress-induced deflections and potential pharmacological interventions.