Food selection by bacterivorous protists:: insight from the analysis of the food vacuole content by means of fluorescence in situ hybridization
Food selection by bacterivorous protists:: insight from the analysis of the food vacuole content by means of fluorescence in situ hybridization
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DOI：
10.1016/j.femsec.2004.12.001
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发表时间：
2005-05-01
影响因子：
4.2
通讯作者：
Simek, K
中科院分区：
文献类型：
--
作者：
Jezbera, J;Horn치k, K;Simek, K
用改良的荧光原位杂交（FISH）方法分析了实验室和自然种群中原生生物食物泡中细菌的猎物组成。在实验室条件下，我们将两种细菌菌株（分别隶属于β-和γ-变形菌门-气单胞菌和荧光假单胞菌）暴露于三种原生生物的放牧：鞭毛虫Bodo saltans和Goniomonas sp.，和纤毛的青蛤属青光眼。这两种鞭毛虫最好能被A. Escherichia优于荧光假单胞菌，而C型青光眼没有表现出明显的偏好。发现B对细菌猎物的消化存在差异。Saltans消化荧光假单胞菌的速度明显快于其他两种原生生物。现场研究是在一个水库进行的，作为一个更大的实验的一部分。我们监测的细菌猎物组成的变化相比，鞭毛虫食物泡摄入的细菌。通过探针HGC 69 a（放线菌）和R-BT 065检测的细菌被鞭毛虫阴性选择。通过探针CF 319 a检测的细菌最初被阳性选择，但沿着细菌细胞大小的时间变化，该趋势在实验期间变为阴性选择。总体而言，我们的原生生物的食物泡含量的分析表明显着的影响鞭毛虫的猎物选择性浮游细菌群落组成。(c)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
A modified fluorescence in situ hybridization (FISH) method was used to analyze bacterial prey composition in protistan food vacuoles in both laboratory and natural populations. Under laboratory conditions, we exposed two bacterial strains (affiliated with beta- and gamma-Proteobacteria - Aeromonas hydrophila and Pseudomonasfluorescens, respectively) to grazing by three protists: the flagellates Bodo saltans and Goniomonas sp., and the ciliate Cyclidium glaucoma. Both flagellate species preferably ingested A. hydrophila over P. fluorescens, while C glaucoma showed no clear preferences. Differences were found in the digestion of bacterial prey with B. saltans digesting significantly faster P. fluorescens compared to two other protists. The field study was conducted in a reservoir as part of a larger experiment. We monitored changes in the bacterial prey composition available compared to the bacteria ingested in flagellate food vacuoles. Bacteria detected by probe HGC69a (Actinobacteria) and R-BT065 were negatively selected by flagellates. Bacteria detected by probe CF319a were initially positively selected but along with a temporal shift in bacterial cell size, this trend changed to negative selection during the experiment. Overall, our analysis of protistan food vacuole content indicated marked effects of flagellate prey selectivity on bacterioplankton community composition. (c) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.