Secretory Leukoprotease Inhibitor (Slpi) Expression Is Required for Educating Murine Dendritic Cells Inflammatory Response Following Quercetin Exposure.
Secretory Leukoprotease Inhibitor (Slpi) Expression Is Required for Educating Murine Dendritic Cells Inflammatory Response Following Quercetin Exposure.
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DOI：
10.3390/nu9070706
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发表时间：
2017-07-06
期刊：
影响因子：
5.9
通讯作者：
Chieppa M
中科院分区：
文献类型：
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作者：
De Santis S;Galleggiante V;Scandiffio L;Liso M;Sommella E;Sobolewski A;Spilotro V;Pinto A;Campiglia P;Serino G;Santino A;Notarnicola M;Chieppa M
树突状细胞（Dendritic cells，DC）具有呈递抗原和启动适应性免疫应答的能力，在抵抗病原感染的免疫防御中发挥着重要作用，同时还能对微生物群中的无害成分产生免疫耐受。食品可以调节肠道DC的炎症状态。在营养衍生产品中，我们研究了槲皮素抑制炎性细胞因子分泌、抗原呈递和DC向引流淋巴结迁移的能力。我们最近确定了Slpi表达作为槲皮素诱导的炎症抑制所需的关键检查点。在这里，我们证明了Slpi-KO DC在槲皮素暴露后分泌一组独特的细胞因子和趋化因子。在体内，富含槲皮素的食物能够诱导回肠中的Slpi表达，而在十二指肠中几乎没有检测到影响。此外，与肠绒毛的基部相比，表达Slpi的细胞在顶端更频繁，这表明槲皮素暴露对于在肠腔中投射潜望镜的DC可能更有效。这些数据表明，富含槲皮素的营养方案可能是有效的抑制炎症综合征影响回结肠道。
Dendritic cells’ (DCs) ability to present antigens and initiate the adaptive immune response confers them a pivotal role in immunological defense against hostile infection and, at the same time, immunological tolerance towards harmless components of the microbiota. Food products can modulate the inflammatory status of intestinal DCs. Among nutritionally-derived products, we investigated the ability of quercetin to suppress inflammatory cytokines secretion, antigen presentation, and DCs migration towards the draining lymph nodes. We recently identified the Slpi expression as a crucial checkpoint required for the quercetin-induced inflammatory suppression. Here we demonstrate that Slpi-KO DCs secrete a unique panel of cytokines and chemokines following quercetin exposure. In vivo, quercetin-enriched food is able to induce Slpi expression in the ileum, while little effects are detectable in the duodenum. Furthermore, Slpi expressing cells are more frequent at the tip compared to the base of the intestinal villi, suggesting that quercetin exposure could be more efficient for DCs projecting periscopes in the intestinal lumen. These data suggest that quercetin-enriched nutritional regimes may be efficient for suppressing inflammatory syndromes affecting the ileo-colonic tract.