Heat-shock response in Schizosaccharomyces pombe cells lacking cyclic AMP-dependent phosphorylation
Heat-shock response in Schizosaccharomyces pombe cells lacking cyclic AMP-dependent phosphorylation
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缺乏环 AMP 依赖性磷酸化的粟酒裂殖酵母细胞的热休克反应
DOI：
10.1007/s002940050183
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发表时间：
1997
期刊：
影响因子：
2.5
通讯作者：
M. Gacto
中科院分区：
文献类型：
--
作者：
J. Fernández;T. Soto;J. Vicente;J. Cansado;M. Gacto
摘要在25°C下生长的裂殖酵母裂殖酵母的对照细胞和pka 1破坏细胞的对数期培养物中测定了48°C下的热敏感性。与对照细胞相比，缺乏蛋白激酶A的细胞表现出相当大的热休克抗性。向对照细胞中添加环磷酸腺苷会导致热休克期间细胞活力进一步下降。这种效果没有观察到与PKA1破坏的细胞，表明cAMP依赖性磷酸化参与调制的热休克反应。当对照或pka 1破坏的细胞在25°C下生长，然后转移到37°C时，它们获得了对随后在48°C下在不存在和存在外源cAMP的情况下处理的热耐受性。在适应性处理过程中抑制蛋白质合成并没有阻止耐热性的发展。然而，在翻译的逮捕显着防止海藻糖积累在控制细胞，但只有轻微影响海藻糖增加PKA1破坏的细胞。这些数据表明，耐热性可能建立在生长的细胞S。通过至少两种独立的翻译后机制：cAMP依赖性蛋白磷酸化的减少和一个迄今未知的过程，可能是独立的海藻糖积累。
Abstract Heat sensitivity at 48°C was determined in log-phase cultures of control and pka1-disrupted cells of the fission yeast Schizosaccharomyces pombe grown at 25°C. Cells devoid of protein kinase A exhibited a considerable heat-shock resistance as compared to control cells. Addition of cAMP to control cells prompted a further decrease in viability during heat shock. This effect was not observed with pka1-disrupted cells, suggesting that cAMP-dependent phosphorylation is involved in modulation of the heat-shock response. When control or pka1-disrupted cells were grown at 25°C and then shifted to 37°C they acquired thermo-tolerance to a subsequent treatment at 48°C both in the absence and in the presence of exogenous cAMP. Inhibition of protein synthesis during the adaptive treatment did not block the development of thermo-tolerance. However, the arrest in translation significantly prevented trehalose accumulation in control cells but only slightly affected trehalose increase in pka1-disrupted cells. These data indicate that heat resistance may be established in growing cells of S. pombe by at least two independent post-translational mechanisms: a decrease in cAMP-dependent protein phosphorylation and a hitherto unknown process which may be independent of trehalose accumulation.
sck1 是裂殖酵母中 cAMP 依赖性蛋白激酶途径缺陷的高拷贝数抑制因子，编码与酿酒酵母 SCH9 激酶同源的蛋白质。
影响因子：
3.3
作者：
Jin,M;Fujita,M;Culley,BM;Apolinario,E;Yamamoto,M;Maundrell,K;Hoffman,CS
通讯作者：
Hoffman,CS