Characterization of Ehrlichia ruminantium replication and release kinetics in endothelial cell cultures
Characterization of Ehrlichia ruminantium replication and release kinetics in endothelial cell cultures
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DOI：
10.1016/j.vetmic.2005.07.012
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发表时间：
2005-09-30
影响因子：
3.3
通讯作者：
Alves, PM
中科院分区：
文献类型：
--
作者：
Marcelino, I;Veríssimo, C;Alves, PM
反刍埃里希体是Heartwater的病原体，Heartwater是一种影响非洲国家和西印度群岛反刍动物的致命蜱传疾病，可用作野生动物和家畜的灭活疫苗。为了提高E.反刍动物生产产量我们表征E.反刍动物生长动力学的复制时间，最大产量，和感染性的高峰。24 h后，E.反刍动物附着/内化和12小时的滞后期，指数增长发生在感染后36-108小时（HPI）内，净增长高达2.2个数量级。最大E.在120 hpi时观察到反刍动物感染性，并将其定义为E.反刍动物培养物本研究表明，考虑到最终产品的质量约束（E。ruminantium疫苗）、E.反刍动物悬液应在113 hpi收获。总的来说，E.反刍动物通过平均感染周期的进展，不仅可以促进E.反刍动物生产产量，具有重要的后果，大规模生产和利用灭活的心水疫苗，但也阐明了一些其他埃利希菌物种的生长机制，在人类和动物健康的影响。(c)2005 Elsevier B. V.保留所有权利。
Ehrlichia ruminantium is the causative agent of Heartwater, a fatal tick-borne disease affecting ruminants in African countries and West Indies and can be used as an inactivated vaccine for wild and domestic animals. In order to improve E. ruminantium production yields we characterize E. ruminantiun growth kinetics in terms of duplication time, maximum production yield, and peak of infectivity. After a 24 h period for E. ruminantium attachment/internalization and a lag phase of 12 h, the exponential growth occurred within 36-108 h post-infection (hpi) with a net increase of up to 2.2 orders of magnitude. Maximum E. ruminantium infectivity was observed at 120 hpi and was defined as the best time of harvesting (TOH) for propagation of E. ruminantium cultures. This study showed that considering the quality constraint of the final product (E. ruminantium vaccine), the E. ruminantium suspension should be harvested at 113 hpi. Overall, the characterization of E. ruminantium progression through the average infection cycle, not only can contribute to the maximization of E. ruminantium production yield, with important consequences for the large scale production and utilization of an inactivated Heartwater vaccine, but also to elucidate growth mechanisms of some of the other ehrlichial species, with emerging impact in human and animal health. (c) 2005 Elsevier B.V. All rights reserved.