The Subgingival Microbiome of Periodontal Pockets With Different Probing Depths in Chronic and Aggressive Periodontitis: A Pilot Study.
The Subgingival Microbiome of Periodontal Pockets With Different Probing Depths in Chronic and Aggressive Periodontitis: A Pilot Study.
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慢性和侵袭性牙周炎不同探诊深度的牙周袋龈下微生物组：一项初步研究
DOI：
10.3389/fcimb.2018.00124
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发表时间：
2018
影响因子：
5.7
通讯作者：
Lu R
中科院分区：
文献类型：
--
作者：
Shi M;Wei Y;Hu W;Nie Y;Wu X;Lu R
牙周炎是一种由牙龈下的牙周病原体定植引起的传染病，它导致牙齿支撑组织的破坏，是对口腔健康的主要威胁，是导致牙齿脱落的最常见原因。本初步研究的目的是表征不同牙周炎患者不同探诊深度牙周袋的龈下细菌多样性。从3例慢性牙周炎（ChP）患者、3例侵袭性牙周炎（AgP）患者和3例牙周健康者（PH）中采集21个龈下菌斑样本。每位牙周炎患者在三个不同探探深度（pd，分别为4 mm、5-6 mm和≥7 mm）的位置取样。使用16S rRNA基因高通量测序和生物信息学分析，我们发现健康和牙周炎样本的牙龈下群落存在很大差异。与此同时，随着ChP囊袋的加深，ChP中胆原体、Fretibacterium、Porphyromonas、Peptococcus、Treponema_2、Defluviitaleaceae_UCG_011、Filifactor和支原体的数量增加，只有杆状杆菌与PD呈负相关。在AgP中，杆状杆菌和克雷伯氏菌与PD呈正相关，而沙雷氏菌、假弧菌、defluviitaleae_ucg_011和Desulfobulbus与PD呈负相关。在这两组中，棒状杆菌的转移方式不同。此外，在龈下菌斑中，同一患者不同PD的口袋样本之间的UniFrac未加权距离明显低于不同患者同一PD类别的口袋样本。这项研究表明，在疾病发展过程中，个别牙齿部位的牙龈下微生物组发生了变化。在相对小样本量的限制下，本初步研究揭示了牙周破坏程度与龈下微生物群之间的动态关系。
Periodontitis is a kind of infectious disease initiated by colonization of subgingival periodontal pathogens, which cause destruction of tooth-supporting tissues, and is a predominant threat to oral health as the most common cause of loss of teeth. The aim of this pilot study was to characterize the subgingival bacterial biodiversity of periodontal pockets with different probing depths in patients with different forms of periodontitis. Twenty-one subgingival plaque samples were collected from three patients with chronic periodontitis (ChP), three patients with aggressive periodontitis (AgP) and three periodontally healthy subjects (PH). Each patient with periodontitis was sampled at three sites, at different probing depths (PDs, one each at 4 mm, 5–6 mm, and ≥ 7 mm). Using 16S rRNA gene high-throughput sequencing and bioinformatic analysis, we found that subgingival communities in health and periodontitis samples largely differed. Meanwhile, Acholeplasma, Fretibacterium, Porphyromonas, Peptococcus, Treponema_2, Defluviitaleaceae_UCG_011, Filifactor, and Mycoplasma increased with the deepening of the pockets in ChP, whilst only Corynebacterium was negatively associated with PD. In AgP, Corynebacterium and Klebsiella were positively associated with PD, while Serratia, Pseudoramibacter, Defluviitaleaceae_UCG_011, and Desulfobulbus were negatively associated with PD. And among these two groups, Corynebacterium shifted differently. Moreover, in subgingival plaque, the unweighted UniFrac distances between samples from pockets with different PD in the same patients were significantly lower than those from pockets within the same PD category from different patients. This study demonstrated the shift of the subgingival microbiome in individual teeth sites during disease development. Within the limitation of the relative small sample size, this pilot study shed new light on the dynamic relationship between the extent of periodontal destruction and the subgingival microbiome.