ULTRASTRUCTURAL-STUDY OF ROTAVIRUS REPLICATION IN CULTURED-CELLS
ULTRASTRUCTURAL-STUDY OF ROTAVIRUS REPLICATION IN CULTURED-CELLS
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DOI：
10.1099/0022-1317-46-1-75
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发表时间：
1980-01-01
影响因子：
3.8
通讯作者：
ESTES, MK
中科院分区：
文献类型：
--
作者：
ALTENBURG, BC;GRAHAM, DY;ESTES, MK
在可重复的条件下，对猴轮状病毒SA 11在许可的[恒河猴肾MA-104细胞] MA 104细胞中的复制周期进行了系统的超微结构分析。在8 h p.i. [感染后]，在粗面内质网（rer）的肿胀囊泡附近观察到小面积的病毒质，其中含有少量80-90 nm的病毒颗粒。在以后的时间，这些夹杂物的大小和数量增加，并含有大量的80-90 nm的颗粒和52-65 nm的颗粒。感染的细胞最终裂解，释放子代病毒。其他细胞学改变包括在溶酶体样体中隔离的病毒颗粒，在核和/或细胞质中的1 - 5 -20 nm管状结构，在rer内的卷曲膜，与病毒颗粒相关的丝束，以及含有1-5个病毒颗粒的线粒体。此外，SA 11复制研究了几个不太允许的细胞系。结果与MA 104细胞的结果相似，只是较少百分比的细胞被有效感染。
A systematic ultrastructural analysis of the replication cycle of the simian rotavirus SA11 in permissive [rhesus monkey kidney MA-104 cells] MA104 cells was performed under reproducible conditions. At 8 h p.i. [post-infection], small areas of viroplasm were seen adjacent to swollen vesicles of the rough endoplasmic reticulum (rer) containing a few 80-90 nm virus particles. At later times, the size and number of these inclusions increased and the rer contained large numbers of the 80-90 nm particles and 52-65 nm particles. Infected cells eventually lysed, releasing progeny virus. Other cytological alterations included virus particles sequestered in lysosome-like bodies, 15-20 nm tubular structures in the nucleus and/or cytoplasm, convoluted membranes within the rer, filament bundles associated with virus particles, and mitochondria containing 1-5 virus particles. In addition, SA11 replication was studied in several less permissive cell lines. The results were similar to those with MA104 cells except that a smaller percentage of the cells were productively infected.