Annexin A2 is a discriminative serological candidate in early hepatocellular carcinoma.
Annexin A2 is a discriminative serological candidate in early hepatocellular carcinoma.
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膜联蛋白 A2 是早期肝细胞癌的鉴别性血清学候选药物
DOI：
10.1093/carcin/bgs372
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发表时间：
2013-03
期刊：
影响因子：
4.7
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Sun Y;Gao G;Cai J;Wang Y;Qu X;He L;Liu F;Zhang Y;Lin K;Ma S;Yang X;Qian X;Zhao X
迄今为止，肝细胞癌（HCC）的有用标志物仍不完全开发。在这里，我们表明，膜联蛋白A2补体甲胎蛋白（AFP），一个广泛使用的肝癌标志物，在血清学监测和早期发现肝癌。首先，使用亚细胞蛋白质组学方法鉴定HCC中差异表达的蛋白质。然后选择膜联蛋白A2用于进一步验证。在肝癌组织中表达率为60.7%（136/224）。用自行建立的夹心酶联免疫吸附试验（ELISA），我们发现在肝癌患者血清中膜联蛋白A2（annexinA2）显著升高（n = 175，中位数，24.75 ng/µ l）与健康人相比（n = 49，中位数，16.69 ng/µ l），良性肿瘤（n = 19，中位数，19.92 ng/µ l），肝炎（n = 23，中位数，6.48 ng/µ l）和肝硬化（n = 51，中位数，7.39 ng/µ l）对照和其他恶性肿瘤（n = 87）。重要的是，在83.2%（79/95）的早期（中位数，24.32ng/μ l）和78.4%（58/74）的AFP阴性（中位数，24.09ng/μ l）患者中观察到膜联蛋白A2浓度升高。与AFP（AUC = 0.73，95%可信区间：0.66 - 0.80）相比，单独的Annexin A2在检测早期HCC时具有更好的受试者工作特征曲线下面积（AUC = 0.79，95%可信区间：0.73 - 0.85）。联合两种标志物显著提高了早期HCC的诊断效率，达到87.4%的灵敏度。此外，在p21-HBx基因敲入转基因小鼠模型中检测了annexin A2在肝癌发生过程中的表达特征。结果表明，膜联蛋白A2的表达在荷肝癌小鼠中大幅升高，与人类样本中的发现一致。结论：annexin A2可能是B型肝炎病毒相关性肝癌的一个独立的血清学候选者，尤其是在血清AFP正常的早期病例中。
To date, the useful markers of hepatocellular carcinoma (HCC) remains incompletely developed. Here, we show that annexin A2 complement alpha-fetoprotein (AFP), a widely used liver cancer marker, in the serologically surveillance and early detection of HCC. First, differentially expressed proteins in HCC were identified using a subcellular proteomic approach. Annexin A2 was then selected for further verification. It was found to be overexpressed in HCC tissues (60.7%, 136/224). Using a self-estabished sandwich enzyme-linked immunosorbent assay, we found that annexin A2 significantly increased in the sera of HCC (n = 175, median, 24.75ng/µl) compared with the healthy (n = 49, median, 16.69ng/µl), benign tumors (n = 19, median, 19.92ng/µl), hepatitis (n = 23, median, 6.48ng/µl) and cirrhosis (n = 51, median, 7.39ng/µl) controls and other malignant tumors (n = 87). Importantly, raised concentrations of annexin A2 were observed in 83.2% (79/95) of early stage (median, 24.32ng/µl) and 78.4% (58/74) of AFP-negative (median, 24.09ng/µl) patients. Annexin A2 alone had a better area under the receiver-operating characteristic curve (AUC = 0.79, 95% confidence interval: 0.73–0.85) in comparison with AFP (AUC = 0.73, 95% confidence interval: 0.66–0.80) in detecting of early stage HCC. Combining both markers notably improved the diagnostic efficiency of early HCC with an achieved sensitivity of 87.4%. Additionally, the expression characteristics of annexin A2 during hepatocarcinogenesis were detected in p21-HBx gene knockin transgenic mice model. The results showed that annexin A2 expression was substantially elevated in HCC-bearing mice, in accordance with the finding in human samples. In conclusion, annexin A2 may be an independent serological candidate for hepatitis B virus–related HCC, especially in the early stage cases with normal serum AFP.
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影响因子：
4.8
作者：
Babiychuk, EB;Monastyrskaya, K;Draeger, A
通讯作者：
Draeger, A
影响因子：
--
作者：
Bandorowicz-Pikula, Joanna;Wos, Marcin;Pikula, Slawomir
通讯作者：
Pikula, Slawomir
DOI：
10.1073/pnas.82.23.7884
发表时间：
1985-01-01
影响因子：
11.1
作者：
GLENNEY, JR;TACK, BF
通讯作者：
TACK, BF
膜联蛋白A2系统和血管稳态。
影响因子：
4
作者：
Flood, Elle C.;Hajjar, Katherine A.
通讯作者：
Hajjar, Katherine A.
影响因子：
14.8
作者：
Hilpert, Kai;Winkler, Dirk F. H.;Hancock, Robert E. W.
通讯作者：
Hancock, Robert E. W.