ROTATION OF THE CELL-NUCLEUS IN LIVING CELLS - A QUANTITATIVE-ANALYSIS
ROTATION OF THE CELL-NUCLEUS IN LIVING CELLS - A QUANTITATIVE-ANALYSIS
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DOI：
10.1111/j.1768-322x.1985.tb00388.x
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
BOUTEILLE, M
中科院分区：
文献类型：
--
作者：
BARD, F;BOURGEOIS, CA;BOUTEILLE, M
在多种细胞类型中都观察到核旋转。然而，定量分析的报道很少，这种现象的意义仍不清楚。为了研究这种类型的核运动，我们对小鼠 L-929 成纤维细胞进行了定量分析，选择这种细胞系是因为它表现出高核旋转活性。使用延时显微电影摄影进行分析。研究了核旋转与其他细胞现象（例如细胞周期和运动）之间的关系。然后，我们研究了姐妹细胞群体的轮换，以研究它是否是由基因决定的。最后，我们对不同培养细胞系的核旋转进行了定性分析。结果表明，核旋转优先发生在围绕有丝分裂的细胞周期阶段。
Nuclear rotation is observed in a variety of cell types. However, few quantitative analyses are reported and the significance of this phenomenon is still unclear. To investigate this type of nuclear movement, we performed a quantitative analysis in mouse L-929 fibroblasts, a cell line chosen since it displays a high nuclear rotational activity. Analyses were performed using time-lapse microcinematography. The relationship between nuclear rotation and other cellular phenomena such as the cell cycle and locomotion were studied. Then, we investigated the rotation in a population of sister cells to study whether it is genetically determined. Finally, we performed a qualitative analysis of nuclear rotation in different cultured cell lines. Results show that nuclear rotations preferentially occur during the phases of the cell cycle which surround mitosis.