Expression of Kin17 and 8-OxoG DNA glycosylase in cells of rodent and quail central nervous system
Expression of Kin17 and 8-OxoG DNA glycosylase in cells of rodent and quail central nervous system
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DOI：
10.1016/s0361-9230(01)00620-7
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发表时间：
2001-09-15
影响因子：
3.8
通讯作者：
Radicella, JP
中科院分区：
文献类型：
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作者：
Araneda, S;Mermet, N;Radicella, JP
Kin17和8-Oxoguanine DNA糖基酶(Ogg1)分别是参与真核细胞中非法重组和DNA修复的蛋白质。为了确定这些蛋白在啮齿动物和鸟类脑中的表达，我们在同一组织切片上结合了Kin17或Ogg1蛋白的免疫细胞化学和胶质纤维酸性蛋白(GFAP，一种星形胶质细胞标记物)的免疫检测。Kin17和Ogg1蛋白均定位于细胞核，广泛分布于鹌鹑和啮齿类动物脑中的神经元种群。然而，GFAP免疫反应阳性细胞从未被Kin17蛋白标记。这在两个被研究物种的神经纤维束、大脑皮层、海马结构、下丘脑区和脑室周围区域都观察到了。将kin17mRNA原位杂交和GFAP免疫检测相结合，证实了上述结果。相反，在鹌鹑和小鼠脑的纤维束、皮质表面、小脑和室管膜表面等脑结构中，GFAP免疫反应细胞常被Ogg1蛋白标记。我们的结果表明，Kin17蛋白(在神经元中观察到)和Ogg1蛋白(在神经元和神经胶质细胞中观察到)的表达在脑系统发育中是保守的。(C)2001年爱思唯尔科学公司。
Kin17 and 8-Oxoguanine DNA glycosylase (Ogg1) are proteins, respectively, involved in illegitimate recombination and DNA repair in eukaryotic cells. To characterize the expression of these proteins in cell types of rodent and avian brains, we combined immunocytochemistry for either Kin17 or Ogg1 proteins with glial fibrillary acidic protein (GFAP, an astrocyte marker) immunodetection on the same tissue section. Both Kin17 and Ogg1 proteins were localized in cell nuclei and were extensively distributed in neuronal populations of quail and rodent brains. However, GFAP-immunoreactive cells were never labeled by Kin17 protein. This was observed in nerve fiber tracts, in the cerebral cortex, the hippocampal formation, the hypothalamic region, and the periventricular regions of the brain of both species studied. These results were confirmed by combining in situ hybridization of kin17 mRNA and GFAP immunodetection. On the contrary, GFAP-immunoreactive cells were often labeled by the Ogg1 protein in brain structures such as fiber tracts, the cortical surface, the cerebellum, and the ependymal surface of both quail and mouse brains. Our results suggest that the expression of the Kin17 protein (observed in neurons) and that of the Ogg1 protein (observed in neurons and glial cells) is conserved in brain phylogeny. (C) 2001 Elsevier Science Inc.