Femtosecond Laser Photodisruption of Porcine Anterior Chamber Angle: An Ex Vivo Study
Femtosecond Laser Photodisruption of Porcine Anterior Chamber Angle: An Ex Vivo Study
复制标题
DOI：
10.3928/15428877-20081101-07
复制
发表时间：
2008-11-01
影响因子：
--
通讯作者：
Gordon, Robert J.
中科院分区：
文献类型：
--
作者：
Liu, Yaoming;Nakamura, Hiroshi;Gordon, Robert J.
背景与目的：飞秒激光是一种很有前途的生物组织微细加工工具。当前研究调查了通过前房角镜透镜输送激光能量在摘除的猪眼中进行飞秒激光消融的非侵入性青光眼治疗的可行性。材料和方法：将新鲜摘除的猪眼在4 ℃下保存在Optisol GS溶液（Bausch & Lomb，罗切斯特，纽约）中长达48小时。使用前房角镜透镜和定制飞秒激光消融系统在角膜的外周内表面尝试光致破裂。使用以0.15数值孔径透镜聚焦的Ti：蓝宝石激光（800-nm波长）进行飞秒激光烧蚀。激光治疗过程通过真实的视频记录，并对病变进行组织学检查。结果：在激光手术过程中，观察到气泡形成，并出现与激光能量和曝光时间呈正相关。当进行组织学检查时，在角膜的外周内表面处始终观察到消融区域。损伤程度随脉冲能量和曝光时间呈非线性增加，在高能量和长时间时表现出饱和。对于一个给定的能量剂量，更少的高能量脉冲比许多低能量pulses.CONCLUSION：目前的研究表明，在前房角激光消融是可行的飞秒激光消融系统，使用房角镜透镜在离体眼睛。结果表明，一种新的非侵入性青光眼激光治疗的潜力。
BACKGROUND AND OBJECTIVE: The femtosecond laser is a promising tool for the micromachining of biological tissues. The current study investigated the feasibility of noninvasive glaucoma therapy by femtosecond laser ablation in enucleated porcine eyes by delivering the laser energy through a gonioscopic lens.MATERIALS AND METHODS: Freshly enucleated porcine eyes were kept in Optisol GS solution (Bausch & Lomb, Rochester, NY) at 4 degrees C for up to 48 hours. Photodisruption was attempted at the peripheral inner surface of the cornea using a gonioscopic lens and a custom femtosecond laser ablation system. Femtosecond laser ablation was performed using a Ti:Sapphire laser (800-nm wavelength) focused with a 0.15 numerical aperture lens. The laser treatment process was recorded by real time video and the lesions were examined histologically.RESULTS: During laser surgery, bubble formation was observed and appeared to be positively related to laser energy and exposure time. When examined histologically, areas of ablation were consistently observed at the peripheral inner surface of the cornea. The extent of the lesion increased nonlinearly with both pulse energy and exposure time, showing saturation at high energy and long times. For a given energy dose, fewer pulses of high energy were more effective than many low energy pulses.CONCLUSION: The current study demonstrates that laser ablation at the anterior chamber angle is feasible by a femtosecond laser ablation system using a gonioscopic lens in ex vivo eyes. The results indicate the potential of a novel noninvasive glaucoma laser therapy.