Host detection by root parasites: Insights from transcription profiles
Host detection by root parasites: Insights from transcription profiles
复制标题
通过根寄生虫进行宿主检测：转录谱的见解
DOI：
10.1142/9789812771506_0003
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发表时间：
2007
影响因子：
20.5
通讯作者：
M. J. Torres
中科院分区：
文献类型：
--
作者：
J. Yoder;Russell L. Reagan;A. Tomilov;N. Tomilova;M. J. Torres
控制成功寄生的遗传因素的详细知识将有助于实施基于生物技术的解决方案来控制独脚金。我们正在研究寄生虫-宿主植物相互作用的遗传学，目的是确定关键的寄生虫基因，这些基因可以成为作物工程抗性的目标。我们的方法是确定在不同阶段的寄生植物基因的转录调控，并评估其在转基因寄生根的功能。在与能源部联合基因组研究所合作，我们正在测序的cDNA丰富的转录本或上调或下调根的兼性寄生虫Triphysaria接触后，与宿主根，宿主根分泌物，或纯化吸器诱导因子。在GenBank和Pscroph数据库（http：//pscroph.尤卡坦半岛。edu）。该网站还提供了同源性搜索的BLAST功能，
The implementation of biotechnology-based solutions for Striga control will be facilitated by a detailed knowledge of the genetic factors controlling successful parasitism. We are investigating the genetics of parasite–host plant interactions with the goal of identifying critical parasite genes that can be targets for engineered resistance in crop plants. Our approach is to identify parasitic plant genes that are transcriptionally regulated during different stages of parasitism and evaluate their functions in transgenic parasite roots. In collaboration with the Department of Energy Joint Genome Institute, we are sequencing cDNAs enriched for transcripts either up or down regulated in roots of the facultative parasite Triphysaria after contact with host roots, host root exudates, or purified haustorium-inducing factors. Over 9000 ESTs representing about 4000 unique genes are available at GenBank and the Pscroph database (http://pscroph. ucdavis. edu). This web site also provides BLAST functions for homology searches and