Telemetry monitoring of pulmonary arterial pressure in freely moving rats
Telemetry monitoring of pulmonary arterial pressure in freely moving rats
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DOI：
10.1152/jappl.1996.81.2.1027
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发表时间：
1996-08-01
影响因子：
3.3
通讯作者：
Clozel, JP
中科院分区：
文献类型：
--
作者：
Hess, P;Clozel, M;Clozel, JP
目前已建立了几种大鼠肺动脉高压模型。然而，到目前为止，很难长时间监测肺动脉压。本研究的目的是开发一种遥测系统，允许慢性监测肺动脉压在自由活动的大鼠。为此，在动物处于麻醉状态时，通过右心室将传感导管植入肺动脉干。该导管连接到植入腹部的发射器。系统的验证分三步进行。首先，我们严格控制遥测系统传输的压力信号，使其准确，并与高保真Millar导管获得的信号相对应。其次，我们评估了植入该系统的慢性后果。第三，我们使用该系统监测肺动脉压在野百合碱诱导的高血压模型，其中波生坦，内皮素受体拮抗剂的影响进行了评估。遥测系统可靠，未导致右心室损伤和/或慢性肺栓塞。经过8-10天的恢复期后，平均肺动脉压稳定。使用该遥测系统，可以跟踪野百合碱引起的肺动脉压升高。在该模型中，波生坦使平均肺动脉压降低13%（P=0.07），表明内皮素在该肺动脉高压模型中的作用。我们的结论是，它是可能的，使用这种遥测系统来监测肺动脉压在自由活动的清醒大鼠。
Several rat models of pulmonary hypertension have been developed. However, up until now it has been difficult to monitor pulmonary arterial pressure for long periods of time. The goal of the present study was to develop a telemetry system allowing chronic monitoring of pulmonary arterial pressure in freely moving rats. For this purpose, while animals were under anesthesia, a sensing catheter was implanted into the pulmonary arterial trunk through the right ventricle. This catheter was connected to an emitter implanted in the abdomen. Validation of the system was performed in three steps. First, acutely, we controlled that the pressure signal transmitted by the telemetry system was accurate and corresponded to a signal obtained with a high-fidelity Millar catheter. Second, we evaluated the chronic consequences of implantation of the system. Third, we used the system to monitor pulmonary arterial pressure in a model of monocrotaline-induced hypertension in which the effects of bosentan, an endothelin-receptor antagonist, were evaluated. The telemetry system was reliable and did not lead to damage of the right ventricle and/or to chronic pulmonary embolism. After a recovery period of 8-10 days, mean pulmonary arterial pressure was stable. With the use of this telemetry system, it was possible to follow the increase of pulmonary arterial pressure induced by monocrotaline. In this model, bosentan decreased mean pulmonary arterial pressure by 13% (P=0.07), suggesting a role of endothelin in this model of pulmonary hypertension. We conclude that it is possible to use this telemetry system to monitor pulmonary arterial pressure in freely moving conscious rats.