MOUSE EXCITATORY AMINO-ACID TRANSPORTER EAAT2 - ISOLATION, CHARACTERIZATION, AND PROXIMITY TO NEUROEXCITABILITY LOCI ON MOUSE CHROMOSOME-2
MOUSE EXCITATORY AMINO-ACID TRANSPORTER EAAT2 - ISOLATION, CHARACTERIZATION, AND PROXIMITY TO NEUROEXCITABILITY LOCI ON MOUSE CHROMOSOME-2
复制标题
DOI：
10.1006/geno.1994.1609
复制
发表时间：
1994-11-15
期刊：
影响因子：
4.4
通讯作者：
AMARA, SG
中科院分区：
文献类型：
--
作者：
KIRSCHNER, MA;COPELAND, NG;AMARA, SG
谷氨酸和天冬氨酸是兴奋性神经递质，与神经系统的许多病理状态有关。细胞外兴奋性氨基酸的积累可能具有细胞毒性，也可能降低癫痫发作阈值。 Na+依赖性高亲和力兴奋性氨基酸转运蛋白（EAAT）的一个重要功能是重新摄取分泌的氨基酸神经递质，可能将细胞外氨基酸浓度维持在无毒且不致癫痫的水平。我们分离了 EAAT2 的小鼠 cDNA，EAAT2 是一种神经递质转运蛋白，与之前克隆的几种高亲和力谷氨酸转运蛋白之一具有广泛的氨基酸序列同源性。小鼠 EAAT2 氨基酸序列与其大鼠和人类同源物分别具有 99% 和 97% 的同一性。它主要在大脑中表达，可能作为神经胶质细胞特异性转运蛋白发挥作用。在种间回交分析中，Eaat2 定位到小鼠 2 号染色体的中心区域，该区域位于调节酒精戒断和癫痫小鼠模型中的神经兴奋性和癫痫发作频率的数量性状基因座附近。 (C) 1994 年学术出版社
Glutamate and aspartate are excitatory neurotransmitters that have been implicated in a number of pathological states of the nervous system. Accumulation of extracellular excitatory amino acids can be cytotoxic and may also lower the seizure threshold in epilepsy. An important function of the Na+-dependent high-affinity excitatory amino acid transporter (EAAT) is the reuptake of secreted amino acid neurotransmitter, possibly maintaining extracellular amino acid concentrations at nontoxic and nonepileptogenic levels. We have isolated the mouse cDNA for EAAT2, a neurotransmitter transporter that shares extensive amino acid sequence homology with one of several previously cloned high-affinity glutamate transporters. The mouse EAAT2 amino acid sequence shares 99 and 97% identity with its rat and human homologues, respectively. It is expressed predominantly in the brain, where it may function as a glia-specific transporter. In an interspecific backcross analysis Eaat2 maps to the central region of mouse chromosome 2, where it is located near quantitative trait loci that modulate neuroexcitability and seizure frequency in mouse models of alcohol withdrawal and epilepsy. (C) 1994 Academic Press, Inc.