Tissue Engineering the Vascular Tree.
Tissue Engineering the Vascular Tree.
复制标题
组织工程血管树。
DOI：
10.1089/ten.teb.2017.0010
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
George,StevenC
中科院分区：
文献类型：
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作者：
Traore,MahamaA;George,StevenC
组织工程和再生医学领域的一个主要障碍仍然是设计和构建用于生物学研究和移植的更大（> 1cm 3）的体外组织。虽然已经成功地创建了三维（3D）毛细血管网络、相对大的动脉（直径>3-5 mm）和最近的小动脉（直径500 μm-1 mm），但是在创建由小动脉（直径40-300 μm）、毛细血管和小静脉组成的活的动态血管网络方面还没有成功。这样的网络将为需要分层血管供应的较大组织和器官提供向所有周围细胞供应营养和氧气的基础。在这项研究中，我们描述了不同的技术和方法，已在努力创造个人的血管和血管网络，以支持工程组织在体内和体外应用。一个特别的重点放在生成血管的平均尺寸，从微米（毛细血管）到一毫米（大动脉）。我们还确定了主要的挑战，同时探索新的机会，以创建整个血管树的模型系统，包括小动脉和小静脉。
A major hurdle in the field of tissue engineering and regenerative medicine remains the design and construction of larger (> 1 cm3)in vitrotissues for biological studies and transplantation. While there has been success in creating three-dimensional (3D) capillary networks, relatively large arteries (diameter >3–5 mm), and more recently small arteries (diameter 500 μm–1 mm), there has been no success in the creation of a living dynamic blood vessel network comprising of arterioles (diameter 40–300 μm), capillaries, and venules. Such a network would provide the foundation to supply nutrients and oxygen to all surrounding cells for larger tissues and organs that require a hierarchical vascular supply. In this study, we describe the different technologies and methods that have been employed in an effort to create individual vessels and networks of vessels to support engineered tissues forin vivoandin vitroapplications. A special focus is placed on the generation of blood vessels with average dimensions that span from microns (capillaries) to a millimeter (large arterioles). We also identify major challenges while exploring new opportunities to create model systems of the entire vascular tree, including arterioles and venules.