A method for parallel, automated, thermal cycling of submicroliter samples
A method for parallel, automated, thermal cycling of submicroliter samples
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DOI：
10.1101/gr.gr1644r
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发表时间：
2001-03-01
期刊：
影响因子：
7
通讯作者：
Davis, RW
中科院分区：
文献类型：
--
作者：
Nakane, J;Broemeling, D;Davis, RW
DNA测序和其他DNA分析过程的大部分成本来自循环测序或PCR期间产生的试剂成本。特别地，在这些方法中使用的酶和染料的高成本通常导致每个样品的热循环成本超过0.50美元。在高通量DNA测序的情况下，这是显著且不必要的花费。新测序仪器的改进的检测效率允许循环测序的反应体积按比例缩小到目前使用的体积的十分之一，导致该方法的成本降低至少10倍。然而，可商购的热循环仪和自动化反应设置装置具有固有的设计限制，这使得处理体积大。
A large fraction of the cost of DNA sequencing and other DNA-analysis processes results from the reagent costs incurred during cycle sequencing or PCR. In particular, the high cost of the enzymes and dyes used in these processes often results in thermal cycling costs exceeding $0.50 per sample. In the case of high-throughput DNA sequencing, this is a significant and unnecessary expense. Improved detection efficiency of new sequencing instrumentation allows the reaction volumes for cycle sequencing to be scaled down to one-tenth of presently used volumes, resulting in at least a 10-fold decrease in the cost of this process. However, commercially available thermal cyclers and automated reaction setup devises have inherent design limitations which make handling volumes of