Neuronal nicotinic receptors in the locust Locusta migratoria -: Cloning and expression
Neuronal nicotinic receptors in the locust Locusta migratoria -: Cloning and expression
复制标题
DOI：
10.1074/jbc.273.29.18394
复制
发表时间：
1998-07-17
影响因子：
4.8
通讯作者：
Maelicke, A
中科院分区：
文献类型：
--
作者：
Hermsen, B;Stetzer, E;Maelicke, A
我们已经确定了5个cDNA克隆，编码烟碱乙酰胆碱受体（nAChR）亚基表达的蝗虫飞蝗的神经系统。其中四个亚基是配体结合α亚基，另一个是结构β亚基。根据北方分析和原位杂交结果，表明至少存在一个以上的nAChR基因，可能编码一个β亚基。LOC α 1、LOC α 3和LOC β 1是最丰富的亚基，并且在成年蝗虫的头神经节和视网膜的相似区域中表达。因为Loc α 3以高亲和力结合α-银环蛇毒素，所以它可以形成同聚nAChR亚型，例如哺乳动物α 7 nAChR Loc α 1和Loc β 1然后可以形成蝗虫脑中的主要异聚nAChR。LOC α 4主要表达于视叶神经节细胞，LOC α 2主要表达于蘑菇体神经元的外周胞体。在蝗虫胚胎的体胃上皮中散布的细胞中还检测到loc α 3 mRNA，表明这种同种型也可能参与神经元兴奋性以外的功能，所有nAChR亚单位基因的转录在孵化前3天左右开始，并持续到整个成体生活。头神经节神经元的电生理记录也表明存在不止一种功能不同的nAChR亚型，我们的研究结果表明存在几个nAChR亚型，至少其中一些异聚体，在这种昆虫物种。
We have identified five cDNA clones that encode nicotinic acetylcholine receptor (nAChR) subunits expressed in the nervous system of the locust Locusta migratoria. Four of the subunits are ligand-binding alpha subunits, and the other is a structural beta subunit. The existence of at least one more nAChR gene, probably encoding a beta subunit, is indicated.Based on Northern analysis and in situ hybridization, the five subunit genes are expressed. loc alpha 1, loc alpha 3, and loc beta 1 are the most abundant subunits and are expressed in similar areas of the head ganglia and retina of the adult locust. Because Loc alpha 3 binds alpha-bungarotoxin with high affinity, it may form a homomeric nAChR subtype such as the mammalian alpha 7 nAChR Loc alpha 1 and Loc beta 1 may then form the predominant heteromeric nAChR in the locust brain. loc alpha 4 is mainly expressed in optic lobe ganglionic cells and loc alpha 2 in peripherally located somata of mushroom body neurons. loc alpha 3 mRNA was additionally detected in cells interspersed in the somatogastric epithelium of the locust embryo, suggesting that this isoform may also be involved in functions other than neuronal excitability, Transcription of all nAChR subunit genes begins approximately 3 days before hatching and continues throughout adult life.Electrophysiological recordings from head ganglionic neurons also indicate the existence of more than one functionally distinct nAChR subtype, Our results suggest the existence of several nAChR subtypes, at least some of them heteromeric, in this insect species.