Reactive Oxygen Species Formation and Cell Death in Catalase-Deficient Tobacco Leaf Discs Exposed to Paraquat
Reactive Oxygen Species Formation and Cell Death in Catalase-Deficient Tobacco Leaf Discs Exposed to Paraquat
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DOI：
10.1007/s12011-011-9244-1
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发表时间：
2012-05-01
影响因子：
3.9
通讯作者：
Patricia Benavides, Maria
中科院分区：
文献类型：
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作者：
Florencia Iannone, Maria;Paola Rosales, Eliana;Patricia Benavides, Maria
在本工作中，烟草（Nicotiana tabaccum L.）在暴露于除草剂百草枯（PQ）后，评价野生型SR 1和转基因CAT 1AS植物（具有基础降低的CAT活性）。超氧阴离子（O（2）（.-））的形成受到抑制，但H2 O2的产生和离子泄漏显着增加，无论是在SR 1或CAT 1AS叶圆片。NADPH氧化酶的活性是组成性的57%，在未处理的转基因叶片比SR 1叶片，大大减少了PQ处理3或21小时。超氧化物歧化酶（SOD）活性显着降低PQ后21小时，显示从70%下降到55%，而过氧化氢酶（CAT）活性下降了平均50%，3小时后的治疗，和90%后21小时，在SR 1和CAT 1AS，分别。同时，在两个暴露时间，与对照SR 1叶圆片相比，未处理的CAT 1AS植物中的总CAT蛋白含量显示降低。PQ降低CAT的表达在SR 1或CAT 1AS植物在3和21小时的治疗。PQ诱导的细胞死亡的机制可能不完全与烟草野生型或转基因植物中ROS的形成和氧化胁迫有关。
In the present work, the response of tobacco (Nicotiana tabaccum L.) wild-type SR1 and transgenic CAT1AS plants (with a basal reduced CAT activity) was evaluated after exposure to the herbicide paraquat (PQ). Superoxide anion (O (2) (.-) ) formation was inhibited at 3 or 21 h of exposure, but H2O2 production and ion leakage increased significantly, both in SR1 or CAT1AS leaf discs. NADPH oxidase activity was constitutively 57% lower in non-treated transgenic leaves than in SR1 leaves and was greatly reduced both at 3 or 21 h of PQ treatment. Superoxide dismutase (SOD) activity was significantly reduced by PQ after 21 h, showing a decrease from 70% to 55%, whereas catalase (CAT) activity decreased an average of 50% after 3 h of treatment, and of 90% after 21 h, in SR1 and CAT1AS, respectively. Concomitantly, total CAT protein content was shown to be reduced in non-treated CAT1AS plants compared to control SR1 leaf discs at both exposure times. PQ decreased CAT expression in SR1 or CAT1AS plants at 3 and 21 h of treatment. The mechanisms underlying PQ-induced cell death were possibly not related exclusively to ROS formation and oxidative stress in tobacco wild-type or transgenic plants.