Plasma cytokines, chemokines and cellular immune responses in pre-school Nigerian children infected with Plasmodium falciparum.
Plasma cytokines, chemokines and cellular immune responses in pre-school Nigerian children infected with Plasmodium falciparum.
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DOI：
10.1186/1475-2875-12-5
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发表时间：
2013-01-07
期刊：
影响因子：
3
通讯作者：
O'Neill SM
中科院分区：
文献类型：
--
作者：
Noone C;Parkinson M;Dowling DJ;Aldridge A;Kirwan P;Molloy SF;Asaolu SO;Holland C;O'Neill SM
疟疾是全世界发病和死亡的主要原因，每年有100多万人死亡，特别是5岁以下儿童。这项研究是第一次检查血浆细胞因子，趋化因子和细胞免疫反应的学龄前尼日利亚儿童感染恶性疟原虫从四个半城市村庄附近的Ile-Ife，Osun州，尼日利亚。从231名儿童（年龄39-73个月）中采集血液，根据每μl血液中的平均恶性疟原虫密度（未感染（n = 89），低密度（<1，000，n = 51），中等密度（1，000 - 10，000，n = 65）和高密度（> 10，000，n = 22））进行分类。检测血浆中IL-12 p70、IL-10、一氧化氮、IFN-γ、TNF、IL-17、IL-4和TGF-β、C-C趋化因子RANTES、MMP-8和TIMP-1。采集外周血单个核细胞并检测先天性免疫细胞标志物（CD 14、CD 36、CD 56、CD 54、CD 11 c和HLA-DR）。在多克隆刺激或用疟疾寄生虫刺激后，对T细胞亚群（CD 4、CD 3和γδTCR）进行IL-10、IFN-γ和TNF的细胞内染色。蛔虫是这些村庄的地方病，考虑到旁观者蠕虫感染的潜在影响，对所有数据进行了分析。使用SPSS 15 for windows分析所有数据，并且在所有测试中，p <0.05被认为是显著的。恶性疟原虫寄生虫血症水平与血浆IL-10呈正相关，与IL-12 p70呈负相关。在疟疾感染者中单核细胞的百分比显著降低，而疟疾寄生虫血症与CD 54+、CD 11 c+和CD 56+细胞群的百分比增加呈正相关。未观察到组间有丝分裂原激活的T细胞群中细胞因子表达的相关性，也未检测到疟疾特异性免疫应答。尽管A.尽管蛔虫在这些村庄是地方病，但数据分析表明，这种蠕虫感染对恶性疟原虫寄生虫病或与恶性疟原虫感染相关的免疫反应没有影响。这些研究结果表明，感染恶性疟原虫的尼日利亚儿童表现出与活动性疟疾感染相关的免疫反应，这些反应与恶性疟原虫寄生虫血症的增加呈正相关。
Malaria is a major cause of morbidity and mortality worldwide with over one million deaths annually, particularly in children under five years. This study was the first to examine plasma cytokines, chemokines and cellular immune responses in pre-school Nigerian children infected with Plasmodium falciparum from four semi-urban villages near Ile-Ife, Osun State, Nigeria. Blood was obtained from 231 children (aged 39–73 months) who were classified according to mean P. falciparum density per μl of blood (uninfected (n = 89), low density (<1,000, n = 51), medium density (1,000-10,000, n = 65) and high density (>10,000, n = 22)). IL-12p70, IL-10, Nitric oxide, IFN-γ, TNF, IL-17, IL-4 and TGF-β, C-C chemokine RANTES, MMP-8 and TIMP-1 were measured in plasma. Peripheral blood mononuclear cells were obtained and examined markers of innate immune cells (CD14, CD36, CD56, CD54, CD11c AND HLA-DR). T-cell sub-populations (CD4, CD3 and γδTCR) were intracellularly stained for IL-10, IFN-γ and TNF following polyclonal stimulation or stimulated with malaria parasites. Ascaris lumbricoides was endemic in these villages and all data were analysed taking into account the potential impact of bystander helminth infection. All data were analysed using SPSS 15 for windows and in all tests, p <0.05 was deemed significant. The level of P. falciparum parasitaemia was positively associated with plasma IL-10 and negatively associated with IL-12p70. The percentage of monocytes was significantly decreased in malaria-infected individuals while malaria parasitaemia was positively associated with increasing percentages of CD54+, CD11c+ and CD56+ cell populations. No association was observed in cytokine expression in mitogen-activated T-cell populations between groups and no malaria specific immune responses were detected. Although A. lumbricoides is endemic in these villages, an analysis of the data showed no impact of this helminth infection on P. falciparum parasitaemia or on immune responses associated with P. falciparum infection. These findings indicate that Nigerian children infected with P. falciparum exhibit immune responses associated with active malaria infection and these responses were positively associated with increased P. falciparum parasitaemia.
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影响因子：
6.4
作者：
Cooper, Philip J.;Moncayo, Ana Lucia;Guadalupe, Irene;Benitez, Susana;Vaca, Maritza;Chico, Martha;Griffin, George E.
通讯作者：
Griffin, George E.
影响因子：
6.4
作者：
Dietmann, Anelia;Helbok, Raimund;Kremsner, Peter G.
通讯作者：
Kremsner, Peter G.
影响因子：
4.4
作者：
Semnani, Roshanak Tolouei;Venugopal, Priyanka Goel;Nutman, Thomas B.
通讯作者：
Nutman, Thomas B.
DOI：
10.1016/j.trstmh.2006.01.005
发表时间：
2006-11-01
影响因子：
2.2
作者：
Jenkins, N. E.;Chakravorty, S. J.;Craig, A. G.
通讯作者：
Craig, A. G.
影响因子：
6.4
作者：
Prakash, D.;Fesel, Constantin;Pied, Sylviane
通讯作者：
Pied, Sylviane