The flavonoid cluercetin inhibits dimethyinitrosamine induced liver damage in rats
The flavonoid cluercetin inhibits dimethyinitrosamine induced liver damage in rats
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DOI：
10.1211/0022357021396
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发表时间：
2003-08-01
影响因子：
3.3
通讯作者：
Moon, JO
中科院分区：
文献类型：
--
作者：
Lee, ES;Lee, HE;Moon, JO
槲皮素是人类饮食中最丰富的黄酮类化合物之一，具有广泛的药理学性质。本研究探讨了槲皮素对二甲基亚硝胺（DMN）诱导的大鼠肝损伤的保护作用。DMN给药导致体重和肝脏重量显著降低。口服槲皮素（每天10 mg/kg，持续4周）可显著预防DMN诱导的体重和肝脏重量减轻，并抑制血清丙氨酸转氨酶、天冬氨酸转氨酶和胆红素水平升高。槲皮素还增加血清白蛋白和肝脏谷胱甘肽水平，降低肝脏丙二醛水平。此外，DMN诱导的羟脯氨酸含量的升高在槲皮素治疗的大鼠中减少，其结果与I型胶原mRNA产生的减少和天狼星红染色的肝组织的组织学分析一致。通过α-平滑肌肌动蛋白染色评估，肝星状细胞活化减少与槲皮素治疗以及转化生长因子β 1表达减少相关。总之，这些结果表明，槲皮素对DMN诱导的肝损伤表现出体内保肝和抗纤维化作用，并表明槲皮素可用于预防肝纤维化的发展。
Quercetin, one of the most abundant flavonoids in human diet has been reported to exhibit a wide range of pharmacological properties. In this study, we investigated the protective effect of quercetin on hepatic injury induced by dimethylnitrosamine (DMN) in rats. Treatment with DMN caused a significant decrease in body and liver weight. Oral administration of quercetin (10 mg kg(-1) daily for 4 weeks) remarkably prevented this DMN-induced loss in body and liver weight and inhibited the elevation of serum alanine transaminase, aspartate transaminase and bilirubin levels. Quercetin also increased serum albumin and hepatic glutathione levels and reduced the hepatic level of malondialdehyde. Furthermore, DMN-induced elevation of hydroxyproline content was reduced in the quercetin treated rats, the result of which was consistent with a reduction in type I collagen mRNA production and histological analysis of liver tissue stained with Sirius red. A reduction in hepatic stellate cell activation, as assessed by a-smooth muscle actin staining, was associated with quercetin treatment as well as a reduction in transforming growth factor-beta1 expression. In conclusion, these results demonstrate that quercetin exhibited in-vivo hepatoprotective and anti-fibrogenic effects against DMN-induced liver injury and suggest that quercetin may be useful in the preventing the development of hepatic fibrosis.