Induction of ocular herpes simplex virus shedding by iontophoresis of epinephrine into rabbit cornea.
Induction of ocular herpes simplex virus shedding by iontophoresis of epinephrine into rabbit cornea.
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通过肾上腺素电离子导入兔角膜诱导眼部单纯疱疹病毒脱落。
DOI：
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发表时间：
1981
影响因子：
4.4
通讯作者：
J. Hill
中科院分区：
文献类型：
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作者：
B. Kwon;L. P. Gangarosa;K. Burch;J. Deback;J. Hill
用0.01%肾上腺素离子导入兔眼内，诱导HSV-1从潜伏感染的兔眼脱落。肾上腺素电离子导入，每次0.8mAh，每次8 min，每日1次，连续3d。通过用眼拭子获得的泪膜中HSV-1的存在来确定脱落。接种病毒后60天进行单侧肾上腺素离子导入，所有病例均导致同侧HSV-1脱落（7/7）。在接种后170至365天期间的选定日期进行双侧肾上腺素离子电渗，导致75%的眼睛（21/28）和100%的家兔（14/14）中HSV-1脱落。所有脱落均在第三次肾上腺素离子电渗治疗后3天内开始。肾上腺素离子导入法诱导的脱落频率显著高于其他方法（pb <0.05）。在所有实验组的每只眼睛的三叉神经节和上级颈神经节的一个或两个共培养外植体中检测到HSV-1，表明所有眼睛都有脱落的可能。总之，肾上腺素离子导入法诱导眼部HSV-1脱落可靠，并在潜伏感染的兔子高频率。此外，我们认为，这种易于重现的病毒脱落模型提供了一个系统，用于研究复发性HSV-1眼部感染的相关因素。
Ocular herpes simplex virus type 1 (HSV-1) shedding from the latently infected rabbit was induced by iontophoresis of 0.01% epinephrine into the eye. The iontophoresis of epinephrine was at 0.8 mAmp for 8 min once a day for 3 consecutive days. Shedding was determined by the presence of HSV-1 in the tear film obtained with eye swabs. Unilateral epinephrine iontophoresis performed 60 days after inoculation of the virus resulted in ipsilateral HSV-1 shedding in all cases (7/7). Bilateral epinephrine iontophoresis performed on selected days during 170 to 365 days after inoculation resulted in HSV-1 shedding in 75% of the eyes (21/28) and 100% of the rabbits (14/14). All shedding was initiated within 3 days after the third treatment with epinephrine iontophoresis. The shedding frequency induced by epinephrine iontophoresis was significantly higher (pb less than 0.05) than that induced by the other methods employed. HSV-1 was detected in one or both cocultivated explants of trigeminal and superior cervical ganglia for every eye in all experimental groups, indicating that all eyes had the potential to shed. In conclusion, epinephrine iontophoresis induced ocular HSV-1 shedding reliably and with a high frequency in the latently infected rabbits. Furthermore, we suggest that this easily reproducible model of viral shedding offers a system for studying the factors involved in recurrent HSV-1 ocular infections.