The Effect of Albumin Bound Polyunsaturated Fatty Acids on Human Platelets
The Effect of Albumin Bound Polyunsaturated Fatty Acids on Human Platelets
复制标题
白蛋白结合多不饱和脂肪酸对人血小板的影响
作者：
S. Holme;J. Brox;Hedvig Krane;A. Nordøy
将富血小板血浆（PRP）与结合至白蛋白的各种多不饱和脂肪酸（PUFA）在22-24° C下孵育24小时，以确定血小板磷脂的FA组成的变化是否可能诱导血小板行为和血小板结构完整性的变化。测试的PUFA是亚油酸（18：2 w 6）*、亚麻酸（18：3 w3）、二高-γ亚麻酸（20：3 w 6）、花生四烯酸（20：4 w 6）和二十碳五烯酸（20：5 w3）。只有PRP与20：3 W 6和20：5 W3孵育，将大量的这些FA掺入血小板磷脂中，并显示出对胶原蛋白聚集和TxB 2产生的抑制。然而，抑制不是由于这些FA掺入磷脂，如通过洗涤和重悬掺入FA的血小板在对照血浆中所观察到的，也不是由于结构完整性的任何变化。这表明，20：3 W 6和20：5 W3对聚集的影响是由于从血小板释放的这些FA的代谢产物。
Summary Platelet rich plasma (PRP) was incubated at 22–24° C for 24 hr with various polyunsaturated fatty acids (PUFA), bound to albumin, to determine whether changes in the FA composition of the platelet phospholipids might induce changes in platelet behavior and in platelet structural integrity. The PUFA tested were linoleic (18:2 w6)*, linolenic (18:3 w3), dihomo-gamma linolenic (20:3 w6), arachidonic (20:4 w6) and eicosapentaenoic acids (20:5 w3). Only PRP incubated with 20:3 w6 and 20:5 w3 incorporated significant amounts of these FA into the platelet phospholipids and showed inhibition of aggregation and TxB2 production with collagen. However, the inhibition was not due to the incorporation of these FA’s into the phospholipids as observed by washing and resuspension of the FA incorporated platelets in control plasma, or to any change in structural integrity. It is suggested that the effect of 20:3 w6 and 20:5 w3 on aggregation is due to metabolites of these FA’s released from the platelets.
高浓度花生四烯酸抑制血小板聚集和释放的机制研究
影响因子：
20.3
作者：
Linder,BL;Goodman,DS
通讯作者：
Goodman,DS
用于输注的血小板在 22 摄氏度下储存：血小板密度和大小、介质 pH 值以及体内输注后活力的相互关系。
DOI：
--
发表时间：
1983
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Holme,S;Murphy,S
通讯作者：
Murphy,S