HPR1 encodes a global positive regulator of transcription in Saccharomyces cerevisiae.
HPR1 encodes a global positive regulator of transcription in Saccharomyces cerevisiae.
复制标题
HPR1 编码酿酒酵母中转录的全局正调节因子。
DOI：
10.1128/mcb.15.3.1698
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发表时间：
1995
影响因子：
5.3
通讯作者：
Christman,MF
中科院分区：
文献类型：
--
作者：
Zhu,Y;Peterson,CL;Christman,MF
The Hpr1 protein has an unknown function, although it contains a region of homology to DNA topoisomerase I. We have found that hpr1 null mutants are defective in the transcription of many physiologically unrelated genes, includingGAL1, HO, ADH1, andSUC2, by using a combination of Northern (RNA) blot analysis, primer extension, and upstream activation sequence-lacZfusions. Many of the genes positively regulated by HPR1 also requireSWI1, SWI2-SNF2, SWI3, SNF5, andSNF6. The transcriptional defect atHOand theCCB::lacZupstream activation sequence in hpr1 mutants is partially suppressed by a deletion of SIN1, which encodes an HMG1p-like protein. Elevated gene dosage of either histones H3 and H4 or H2A and H2B results in a severe growth defect in combination with anhpr1null mutation. However, increased gene dosage of all four histones simultaneously restores near-normal growth inhpr1mutants. Altered in vivo Dam methylase sensitivity is observed at twoHPR1-dependent promoters (GAL1andSUC2). Most of the Hpr1 protein present in the cell is in a large complex (106Da) that is distinct from the SWI-SNF protein complex. We propose thatHPR1affects transcription and recombination by altering chromatin structure.
