IDENTIFICATION OF A RAT-LIVER NUCLEAR-PROTEIN THAT BINDS TO THE ENHANCER CORE ELEMENT OF 3 ANIMAL VIRUSES
IDENTIFICATION OF A RAT-LIVER NUCLEAR-PROTEIN THAT BINDS TO THE ENHANCER CORE ELEMENT OF 3 ANIMAL VIRUSES
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DOI：
10.1101/gad.1.2.133
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发表时间：
1987-04-01
影响因子：
10.5
通讯作者：
MCKNIGHT, SL
中科院分区：
文献类型：
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作者：
JOHNSON, PF;LANDSCHULZ, WH;MCKNIGHT, SL
本报告描述了从大鼠肝脏中鉴定和纯化一种核蛋白，该蛋白选择性地结合与几种动物病毒增强子相关的DNA序列。通过四步生化分馏，采用直接dna酶I足迹法追踪其结合活性。这些步骤导致鉴定出一个多肽物种，其表观分子量为20 kD，这说明了增强子的结合活性。DNase I和硫酸二甲酯足迹测定法用于检测纯化蛋白与SV40、小鼠肉瘤病毒和多瘤病毒相关的增强元件结合的方式。这些试验的结果表明，在20-kD蛋白和每个病毒增强子之间建立的初始相互作用是通过称为增强子核心同源性的共同DNA序列发生的。
This report describes the identification and purification of a nuclear protein from rat liver that binds selectively to DNA sequences associated with several animal virus enhancers. The binding activity was tracked by direct DNase I footprinting through four steps of biochemical fractionation. These procedures led to the identification of a polypeptide species exhibiting an apparent molecular weight of 20 kD that accounts for enhancer binding activity. DNase I and dimethyl sulfate footprinting assays were used to examine the manner in which the purified protein binds to enhancer elements associated with SV40, murine sarcoma virus, and polyoma virus. The results of these assays indicate that the initial interaction established between the 20-kD protein and each viral enhancer occurs via a common DNA sequence known as the enhancer core homology.