Metabonomic study on 'Kidney-Yang Deficiency syndrome' and intervention effects of Rhizoma Drynariae extracts in rats using ultra performance liquid chromatography coupled with mass spectrometry
Metabonomic study on 'Kidney-Yang Deficiency syndrome' and intervention effects of Rhizoma Drynariae extracts in rats using ultra performance liquid chromatography coupled with mass spectrometry
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DOI：
10.1016/j.talanta.2010.09.026
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发表时间：
2011-01-15
期刊：
影响因子：
6.1
通讯作者：
Li, Famei
中科院分区：
文献类型：
--
作者：
Lu, Xiumei;Xiong, Zhili;Li, Famei
本研究旨在探讨大剂量氢化可的松所致肾阳虚证的代谢组学特征及传统中药骨碎补对肾阳虚证的治疗作用。建立了一种基于超高效液相色谱-质谱联用（UPLC/MS）的尿液代谢组学方法。主成分分析（PCA）显示模型组（氢化可的松诱导组）与给药前组（氢化可的松诱导前组）代谢谱有显著性差异。骨碎补治疗组（氢化可的松诱导大鼠模型后，给予骨碎补乙醇提取物）在第3 ~ 15天呈现时间依赖性的消退趋势，表明骨碎补对“肾阳虚证”大鼠具有时间依赖性的恢复作用。已鉴定出一些显著变化的代谢物，如苯丙氨酸、苯乙酰甘氨酸、N-2-琥珀酰-L-鸟氨酸、L-脯氨酸、肌酐、马尿酸和柠檬酸。这些生化变化与能量代谢、氨基酸代谢及肠道菌群失调有关，有助于进一步认识肾阳虚证及骨碎补的治疗机理。研究表明，代谢组学方法是研究中医证候理论本质和中医药疗效机制的有效工具。(C)2010 Elsevier B. V.保留所有权利。
This paper was designed to study metabonomic characters of the 'Kidney-Yang Deficiency syndrome' induced by high dose of hydrocortisone and the therapeutic effects of Rhizoma Drynariae, classic traditional Chinese medicine (TCM) in treating the syndrome. A urinary metabonomics method based on ultra-performance liquid chromatography coupled with mass spectrometry (UPLC/MS) was developed. The significant difference in metabolic profiling was observed from model group (hydrocortisone-induced group) compared with the pre-dose group (rats before hydrocortisone inducing) by using the principal components analysis (PCA). The time-dependent regression tendency in Rhizoma Drynariae treatment group (hydrocortisone-induced rats followed by being administered with Rhizoma Drynariae ethanol extracts) from day 3 to 15 was obtained, indicating the time-dependent recovery effect of Rhizoma Drynariae on 'Kidney-Yang Deficiency syndrome' rats. Some significantly changed metabolites like phenylalanine, phenylacetylglycine, N-2-succinyl-L-ornithine, L-proline, creatinine, hippurate and citrate have been identified. These biochemical changes are related to the disturbance in energy metabolism, amino acid metabolism and gut microflora, which are helpful to further understand the 'Kidney-Yang Deficiency syndrome' and the therapeutic mechanism of Rhizoma Drynariae. The work shows that the metabonomics method is a valuable tool for studying the essence of Chinese medicine's syndrome theory and therapeutic effect mechanism of TCM. (C) 2010 Elsevier B.V. All rights reserved.