Acetone-butanol-ethanol production from Kraft paper mill sludge by simultaneous saccharification and fermentation
Acetone-butanol-ethanol production from Kraft paper mill sludge by simultaneous saccharification and fermentation
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DOI：
10.1016/j.biortech.2015.10.102
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发表时间：
2016-01-01
影响因子：
11.4
通讯作者：
Lee, Yoon Y.
中科院分区：
文献类型：
--
作者：
Guan, Wenjian;Shi, Suan;Lee, Yoon Y.
研究了纸浆和造纸工业的固体废弃物造纸厂污泥（PS）作为同时糖化发酵（SSF）生产丙酮-丁醇-乙醇（ABE）的原料。醋酸丁酸梭菌发酵造纸污泥需要去除PS中的部分灰分以提高其酶消化率。酶解被发现是SSF的限速步骤。在固载率为6.3 ~ 7.4%、酶载率为10 ~ 15 FPU/g-葡聚糖的条件下，脱灰PS的SSF共产生16.4 ~ 18.0 g/L的ABE溶剂，最终溶剂得率达到0.27 g/g糖。造纸污泥ABE发酵不需要预处理和pH控制，是生产丁醇的理想原料。结果表明，废纸污泥的利用既要考虑CaCO3在发酵过程中的缓冲作用，也要考虑其对酶解的抑制作用。Elsevier Ltd.出版。
Paper mill sludge (PS), a solid waste from pulp and paper industry, was investigated as a feedstock for acetone-butanol-ethanol (ABE) production by simultaneous saccharification and fermentation (SSF). ABE fermentation of paper sludge by Clostridium acetobutylicum required partial removal of ash in PS to enhance its enzymatic digestibility. Enzymatic hydrolysis was found to be a rate-limiting step in the SSF. A total of 16.4-18.0 g/L of ABE solvents were produced in the SSF of de-ashed PS with solid loading of 6.3-7.4% and enzyme loading of 10-15 FPU/g-glucan, and the final solvent yield reached 0.27 g/g sugars. No pretreatment and pH control were needed in ABE fermentation of paper sludge, which makes it an attractive feedstock for butanol production. The results suggested utilization of paper sludge should not only consider the benefits of buffering effect of CaCO3 in fermentation, but also take into account its inhibitory effect on enzymatic hydrolysis. Published by Elsevier Ltd.