ACTIVITIES OF HERPES-SIMPLEX VIRUS TYPE-1 (HSV-1) ICP4 GENES SPECIFYING NONSENSE PEPTIDES
ACTIVITIES OF HERPES-SIMPLEX VIRUS TYPE-1 (HSV-1) ICP4 GENES SPECIFYING NONSENSE PEPTIDES
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DOI：
10.1093/nar/15.11.4491
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发表时间：
1987-06-11
影响因子：
14.9
通讯作者：
SCHAFFER, PA
中科院分区：
文献类型：
--
作者：
DELUCA, NA;SCHAFFER, PA
在所有可能的阅读框中含有翻译终止密码子的合成寡核苷酸接头被插入编码单纯疱疹病毒1型（HSV-1）立即早期调节蛋白ICP 4的克隆基因的各个位置。经测定，ICP 4基因的氨基末端60%足以反式诱导胸苷激酶启动子-CAT嵌合体（pTKCAT）和ICP 4启动子-CAT嵌合体（pIE 3CAT）的负调控;然而，它在补充ICP 4缺失突变体方面相对低效。氨基末端的90个氨基酸似乎不是感染性所需的，如在氨基酸12处含有接头插入的突变病毒的复制能力所反映的。从突变体病毒表达的ICP 4分子的大小与对应于推导的ICP 4氨基酸序列的氨基酸90的下一个甲硫氨酸密码子处的翻译重启一致。
Synthetic oligonucleotide linkers containing translational termination codons in all possible reading frames were inserted at various positions in the cloned gene encoding the herpes simplex virus type 1 (HSV-1) immediate-early regulatory protein, ICP4. It was determined that the amino-terminal 60 percent of the ICP4 gene was sufficient for trans-induction of a thymidine kinase promoter-CAT chimera (pTKCAT) and negative regulation of an ICP4 promoter-CAT chimera (pIE3CAT); however, it was relatively inefficient in complementing an ICP4 deletion mutant. The amino-terminal ninety amino acids do not appear to be required for infectivity as reflected by the replication competence of a mutant virus containing a linker insertion at amino acid 12. The size of the ICP4 molecule expressed from the mutant virus was consistent with translational restart at the next methionine codon corresponding to amino acid 90 of the deduced ICP4 amino acid sequence.