Direct evidence for increase in excitatory amino acids release during mossy fiber LTP in rat hippocampal slices as revealed by the patch sensor methods
Direct evidence for increase in excitatory amino acids release during mossy fiber LTP in rat hippocampal slices as revealed by the patch sensor methods
复制标题
贴片传感器方法揭示了大鼠海马切片苔藓纤维 LTP 期间兴奋性氨基酸释放增加的直接证据
DOI：
10.1016/s0304-3940(97)13474-7
复制
发表时间：
1997
影响因子：
2.5
通讯作者：
M. Satoh
中科院分区：
文献类型：
--
作者：
T. Maeda;S. Kaneko;A. Akaike;M. Satoh
我们之前已经建立了一个斑贴传感器系统，用于检测单一电刺激引起的海马片兴奋性氨基酸(EAA)的溢出。我们在大鼠海马片上同时记录了斑片传感器电流和全细胞电流。苔藓纤维强直可使CA3锥体细胞记录到的兴奋性突触后电流(EPSC)产生长时程增强(LTP)，并使位于透明层的贴片传感器记录到的单个电刺激诱发的膜片电流幅度增加。在Schaffer侧支-CA1突触中，EPSC的LTP伴随着AMPA诱发的全细胞电流的增加，但不伴随辐射层的斑块电流的变化。这些结果表明苔藓纤维LTP中EAA的释放增加，而CA1 LTP中的EAA释放增加。
We had previously established a patch sensor system for detecting overflow of excitatory amino acids (EAA) from hippocampal slices induced by a single electric stimulus. We performed simultaneous recordings of patch sensor current and whole-cell current in rat hippocampal slices. Tetanus of mossy fibers produced long-term potentiation (LTP) of excitatory postsynaptic current (EPSC) recorded from a CA3 pyramidal cell with an increase in single electric stimulus-evoked patch current amplitude recorded with a patch sensor positioned in the stratum lucidum. In Schaffer collateral–CA1 synapses, however, LTP of EPSC was accompanied by an increase in AMPA-evoked whole-cell current, but not with change in patch current in the stratum radiatum. These results indicate that EAA release is increased during mossy fiber LTP but not CA1 LTP.