In Vitro Study of Directly Bioprinted Perfusable Vasculature Conduits.
In Vitro Study of Directly Bioprinted Perfusable Vasculature Conduits.
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DOI：
10.1039/c4bm00234b
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发表时间：
2015-01
影响因子：
6.6
通讯作者：
Ozbolat IT
中科院分区：
文献类型：
--
作者：
Zhang Y;Yu Y;Akkouch A;Dababneh A;Dolati F;Ozbolat IT
创建三维（3D）厚组织的能力仍然是一个主要的组织工程挑战。它需要发展合适的血管供应以进行有效的介质交换。当构建具有高代谢活性的厚功能组织和/或器官（例如心脏、肝脏和胰腺）时，特别需要集成的脉管系统网络。在这项工作中，人脐静脉平滑肌细胞（HUVSMCs）被封装在海藻酸钠和打印的血管导管的形式使用同轴沉积系统。进行了详细的调查，以了解印刷导管的脱水，溶胀和降解特性。此外，由于灌注，渗透和机械性能是天然血管的独特特性，对于打印导管，这些性能也在这项工作中进行了探索。结果表明，在导管中包封的细胞具有良好的增殖活性，并且它们的活力在长时间的体外培养期间增加。组织学观察发现，长期培养的细胞性血管管道周围及管腔表面均有平滑肌基质和胶原沉积。
The ability to create three dimensional (3D) thick tissues is still a major tissue engineering challenge. It requires the development of a suitable vascular supply for an efficient media exchange. An integrated vasculature network is particularly needed when building thick functional tissues and/or organs with high metabolic activities, such as the heart, liver and pancreas. In this work, human umbilical vein smooth muscle cells (HUVSMCs) were encapsulated in sodium alginate and printed in the form of vasculature conduits using a coaxial deposition system. Detailed investigations were performed to understand the dehydration, swelling and degradation characteristics of printed conduits. In addition, because perfusional, permeable and mechanical properties are unique characteristics of natural blood vessels, for printed conduits these properties were also explored in this work. The results show that cells encapsulated in conduits had good proliferation activities and that their viability increased during prolonged in vitro culture. Deposition of smooth muscle matrix and collagen was observed around the peripheral and luminal surface in long-term cultured cellular vascular conduit through histology studies.