Gene expression in human cumulus cells: one approach to oocyte competence
Gene expression in human cumulus cells: one approach to oocyte competence
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DOI：
10.1093/humrep/dem336
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发表时间：
2007-12-01
影响因子：
6.1
通讯作者：
Royere, D.
中科院分区：
文献类型：
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作者：
Feuerstein, P.;Cadoret, V.;Royere, D.
背景：卵母细胞与卵丘细胞之间的对话是卵母细胞成熟的必要条件。本研究设计了一个前瞻性的实验室研究项目，根据单个卵母细胞核成熟度和发育能力，评估排卵前卵泡卵胞浆内单精子注射前收获的卵丘细胞中特定基因的转录。选择基因是因为它们的表达由LH峰[类固醇生成急性调节蛋白（星星）、环氧合酶2（COX 2或PTGS 2）、双调蛋白（AREG）]诱导，或者因为它们参与卵母细胞促性腺激素代谢[硬脂酰辅酶A去饱和酶I和5（SCD 1和SCD 5）]或间隙连接（连接蛋白43（CX 43或GJA 1）]。方法：采用实时荧光定量PCR技术检测卵丘细胞mRNA水平。结果：减数分裂恢复后，除Cx43外，所有研究基因的表达水平均升高。因此，核成熟与卵丘细胞增加的星星、COX 2、AREG、SCD 1和SCD 5的表达相关。当只考虑卵丘与中期II卵母细胞，基因表达是独立的形态状态在第2天。相比之下，转录水平较低，并在更窄的范围内分布在卵丘包围卵母细胞实现囊胚发育在第5/6天比卵丘包围卵母细胞不能发展超过胚胎阶段。结论：这些基因的低表达与从胚胎到囊胚阶段的进一步发育潜能有关。
BACKGROUND: Dialogue between the oocyte and cumulus cells is essential for oocyte maturation. A prospective laboratory research project was designed to evaluate transcription of specific genes in cumulus cells harvested before intracytoplasmic sperm injection from pre-ovulatory follicles, according to individual oocyte nuclear maturity and developmental competence. Genes were chosen because their expression was induced by the LH peak [Steroidogenic Acute Regulatory protein (STAR), Cyclooxygenase 2 (COX2 or PTGS2), Amphiregulin (AREG)] or because they were involved in oocyte lipidic metabolism [Stearoyl-Coenzyme A Desaturase I and 5 (SCD1 and SCD5)] or in gap-junctions (Connexin 43 (CX43 or GJA1)]. METHODS: mRNA levels in cumulus cells were assessed by real-time PCR. RESULTS: Expression levels of all genes investigated, except Cx43, were increased after resumption of meiosis. Nuclear maturation was thus associated with increased expression of STAR, COX2, AREG, SCD1 and SCD5 by cumulus cells. When considering only cumulus associated with metaphase II oocytes, gene expression was independent of morphological status at Day 2. In contrast, transcript levels were lower and distributed over a narrower range in cumulus enclosing oocytes achieving blastocyst development at Day 5/6 than in cumulus enclosing oocytes unable to develop beyond the embryo stage. CONCLUSION: Further developmental potential from embryo to blastocyst stage was associated with lower expression in a narrow range for these genes.