BIVARIATE FLOW KARYOTYPING OF HUMAN-CHROMOSOMES - EVALUATION OF VARIATION IN HOECHST-33258 FLUORESCENCE, CHROMOMYCIN-A3 FLUORESCENCE, AND RELATIVE CHROMOSOMAL DNA CONTENT
BIVARIATE FLOW KARYOTYPING OF HUMAN-CHROMOSOMES - EVALUATION OF VARIATION IN HOECHST-33258 FLUORESCENCE, CHROMOMYCIN-A3 FLUORESCENCE, AND RELATIVE CHROMOSOMAL DNA CONTENT
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DOI：
10.1002/cyto.990120613
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
ATEN, JA
中科院分区：
文献类型：
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作者：
BOSCHMAN, GA;RENS, W;ATEN, JA
将19个健康个体的Hoechst 33258和chromomycin A3荧光双变量分布中染色体峰位的总变异与从单个细胞系中分别制备的23个染色体双变量分布的实验变异进行比较。Hoechst和chromomycin荧光和相对染色体DNA含量的实验变化是通过几天的实验来确定的。由时间贡献的额外方差与每日方差相同。研究了从二元峰位置计算染色体相对DNA含量的准确性。使用最小二乘法拟合相对DNA含量的分布，分别从同一细胞系的染色体单变量和双变量流分析中获得。总的来说，DNA的含量匹配得很好，但在一些染色体上发现了差异，统计上可识别为5%的水平。从双变量峰位置计算的19个正常个体染色体的平均相对染色体DNA含量与其他研究者发表的估计呈线性关系。
The total variation of chromosome peak positions, in bivariate distributions of Hoechst 33258 and chromomycin A3 fluorescence of 19 healthy individuals, was compared with the experimental variation, determined from 23 bivariate distributions of chromosomes prepared separately from a single cell lineage. The experimental variation in Hoechst and chromomycin fluorescence and the relative chromosomal DNA content were determined from experiments performed over several days. The additional variance contributed by time was the same as the daily variance. The accuracy by which the relative chromosomal DNA content can be calculated from bivariate peak positions was investigated. A least squares method was used to fit the distributions of relative DNA content, obtained, respectively, from mono- and bivariate flow analyses of chromosomes from the same cell lineage. In general the DNA contents match quite well, but for a few chromosomes a difference was found, statistically discernible at the 5% level. The average relative chromosomal DNA content of the chromosomes from the 19 normal individuals, calculated from bivariate peak positions, showed a linear relation with the estimates published by other investigators.