Inflammatory molecules in the tears of patients with keratoconus
Inflammatory molecules in the tears of patients with keratoconus
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DOI：
10.1016/j.ophtha.2004.11.050
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发表时间：
2005-04-01
期刊：
影响因子：
13.7
通讯作者：
Durán, JA
中科院分区：
文献类型：
--
作者：
Lema, I;Durán, JA
目的：确定圆锥角膜患者眼泪中一组炎症分子和基质金属蛋白酶的水平。设计：一项前瞻性病例对照研究。参与者：28 名患者（每人 1 只眼睛）在此期间在西班牙圣地亚哥孔波斯特拉的加莱戈德奥夫塔莫洛西亚研究所诊断为圆锥角膜。 2001年9月至2002年6月，对20名正常对照者（每人1只眼）进行研究。方法：对圆锥角膜患者进行常规检查，并读取角膜曲率读数来监测角膜扩张程度。每只眼通过毛细管流收集 15 微升泪液。 主要指标：细胞因子（白细胞介素 4 [IL-4]、IL-6、IL-10 和肿瘤坏死因子 a [TNF-α]）、细胞粘附分子（细胞间粘附分子 1 和血管细胞粘附分子 1）和细胞因子的浓度。 通过酶联免疫吸附测定法测量基质金属蛋白酶 9 (MMP-9)。 结果：圆锥角膜患者最初的 IL-6 水平显着升高（6.7 [4.8-10.8] pg/ml，对照受试者为 2.2 [1.0-4.1] pg/ml [P < 0.0001]），TNF-a (3.8 [2.9-14.4] pg/ml 对比对照受试者中的 1.8 [1.5-2.3] pg/ml [P < 0.0001]），以及 MMP-9（66.5 [49.2-139.3] ng/ml 对比对照受试者中的 6.1 [3.9-8.3] ng/ml。增加的程度被发现与圆锥角膜。结论。 白细胞介素6、TNF-α和MMP-9在圆锥角膜患者的泪液中过度表达，表明圆锥角膜的发病机制可能涉及慢性炎症事件。眼科学2005; 112:654-659 alpha 2005 年，美国眼科学会。
Purpose: To determine levels of a panel of inflammatory molecules and matrix metal loprotei nases in the tears of patients with keratoconus.Design: A prospective, case-control study.Participants: Twenty-eight patients (1 eye from each) diagnosed with keratoconus at the Instituto Galego de Oftalmoloxia, Santiago cle Compostela, Spain, during the period from September 2001 to June 2002, and 20 normal control subjects (1 eye each) were studied.Methods: Patients with keratoconus were examined in a routine fashion, and keratometric readings were taken to monitor the degree of ectasia. Fifteen microliters of tears was collected by capillary flow from each eye.Main Outcome Measures: The concentrations of cytokines (interleukin-4 [IL-4], IL-6, IL-10, and tumor necrosis factor a [TNF-alpha]), cell adhesion molecules (intercellular adhesion molecule 1 and vascular cell adhesion molecule 1), and matrix metal loprotei nase 9 (MMP-9) were measured by enzyme-linked immunoadsorbent assay.Results: Patients with keratoconus initially had significantly higher levels of IL-6 (6.7 [4.8-10.8] pg/ml vs. 2.2 [1.0-4.1] pg/ml in control subjects [P < 0.0001]), TNF-a (3.8 [2.9-14.4] pg/ml vs. 1.8 [1.5-2.3] pg/ml in control subjects [P < 0.0001]), and MMP-9 (66.5 [49.2-139.3]ng/ml vs. 6.1 [3.9-8.3] ng/ml in control subjects. The extent of the increase was found to be associated with the severity of keratoconus.Conclusions. Interleukin-6, TNF-alpha, and MMP-9 are overexpressed in the tears of patients with keratoconus, indicating that the pathogenesis of keratoconus may involve chronic inflammatory events. Ophthalmology 2005; 112:654-659 alpha 2005 by the American Academy of Ophthalmology.