Mouse Models of Respiratory Syncytial Virus Infection.
Mouse Models of Respiratory Syncytial Virus Infection.
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DOI：
10.1007/978-1-0716-2364-0_2
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发表时间：
2022
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中科院分区：
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呼吸道合胞病毒(RSV)感染在婴幼儿中造成相当大的死亡率和发病率。呼吸道合胞病毒感染似乎引起以Th1型细胞和Th2型细胞为特征的混合免疫反应。这种免疫反应，以及RSV感染引起的毛细支气管炎、喘息和呼吸窘迫等临床特征，呈现出与哮喘的许多特征相似的特征，并导致对严重RSV感染和哮喘之间的联系进行了研究。小鼠的RSV感染是一个强大而有用的工具，可以激发Th2型驱动的免疫反应，从机制上深入了解严重的RSV感染。在这里，我们介绍了几种用于繁殖和纯化RSV、感染RSV的小鼠以及分析RSV感染小鼠样本的材料和方法。
Respiratory syncytial virus (RSV) infection causes considerable mortality and morbidity in infants and young children. RSV infection appears to elicit a mixed immune response characterized by both Th1-type cells and Th2-type cells. This immune response, along with clinical features such as bronchiolitis, wheezing, and respiratory distress caused by RSV infection, presents similarly to many features of asthma and has led to an investigation into the link between severe RSV infection and asthma. RSV infection in mice is a powerful and useful tool for eliciting a Th2-type-driven immune response, lending mechanistic insight into severe RSV infection. Here we present several materials and methods used for propagating and purifying RSV, infecting mice with RSV, and analyzing samples from RSV-infected mice.