A polarized localization of amino acid/carnitine transporter B0,+ (ATBO0,+) in the blood-brain barrier
A polarized localization of amino acid/carnitine transporter B0,+ (ATBO0,+) in the blood-brain barrier
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DOI：
10.1016/j.bbrc.2008.08.122
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发表时间：
2008-11-14
影响因子：
3.1
通讯作者：
Nalecz, Katarzyna A.
中科院分区：
文献类型：
--
作者：
Czeredys, Magdalena;Mysiorek, Caroline;Nalecz, Katarzyna A.
脑毛细血管内皮细胞控制着许多亲水性化合物的摄取和流出，这是由于高度特化的转运蛋白通常以极化的方式定位。在星形胶质细胞上方过滤器上培养的牛脑毛细血管内皮细胞（BBCEC）（体外血脑屏障模型）中，研究了Na+和Cl-依赖性氨基酸和肉碱转运体Br-0、Br-+ (ATBu(0,+))的定位。用和B-0、B-+抗体进行免疫印迹和三维免疫细胞化学分析，证实了这种转运体的存在，并且它普遍与p糖蛋白共定位，即在根尖侧。通过顶膜摄取亮氨酸对2-氨基双环-[2.2.1]-庚烷-2-羧酸（BCH）、d -丝氨酸以及钠和氯化物替代的敏感性证实了ATB（0,+）的功能，并提示ATB（0,+）在控制氨基酸和肉毒碱向大脑的传递方面具有重要的生理作用。(C) 2008爱思唯尔公司版权所有。
Brain capillary endothelial cells control the uptake and efflux from the brain of many hydrophilic compounds due to highly specialized transporters often localized in a polarized way. Localization of Na+- and Cl--dependent amino acid and carnitine transporter Br-0,Br-+ (ATBu(0,+)) was studied in a co-culture of bovine brain capillary endothelial cells (BBCEC) grown on filters above astrocytes (an in vitro blood-brain barrier model). Immunoblotting and three-dimensional immunocytochemistry analysis with and B-0,B-+ antibodies demonstrated the presence of this transporter and its prevalent co-localization with P-glycoprotein i.e. at the apical side. The sensitivity of leucine uptake through the apical membrane to 2-aminobicyclo-[2.2.1]-heptane-2-carboxylic acid (BCH), D-serine as well as sodium and chloride replacement confirm the functioning of ATB(0,+) and suggests an important physiological role of ATB(0,+) in controlling the delivery of amino acids and carnitine to the brain. (C) 2008 Elsevier Inc. All rights reserved.