Effect of temperature on geminivirus-induced RNA silencing in plants
Effect of temperature on geminivirus-induced RNA silencing in plants
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DOI：
10.1104/pp.105.066563
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发表时间：
2005-08-01
期刊：
影响因子：
7.4
通讯作者：
Fauquet, CM
中科院分区：
文献类型：
--
作者：
Chellappan, P;Vanitharani, R;Fauquet, CM
短干扰RNA（Short-interfering RNA，siRNA）是转录后基因沉默（posttranscriptional gene silencing，PTGS）的分子标记，是干扰植物和动物基因表达和抗病毒感染的有力工具。在这里，我们报告了温度对双生病毒诱导的基因沉默的影响，通过量化病毒衍生的siRNA，并通过评估其分布沿着病毒基因组分离的5种木薯双生病毒在木薯（木薯，Crantz）和烟草本塞姆安娜。木薯双生病毒诱导的RNA沉默通过将温度从25摄氏度升高到30摄氏度而增加，并且出现症状较少的新发育的叶子，而与病毒的性质无关。因此，非恢复型双生病毒在高温下表现出恢复型病毒的特性。接下来，我们在三个温度（25 ℃、25 ℃-30 ℃和30 ℃）下评估了病毒衍生的siRNA在相应病毒基因组上的分布。对于恢复型病毒，在较高温度下病毒诱导的PTGS期间，siRNA以适度较高的水平积累，并且siRNA群体沿着病毒基因组的分布没有变化。对于非恢复型病毒，siRNA的积累水平显著高于在高温下用恢复型病毒感染所观察到的水平。如RNA病毒所确定的，温度影响单链DNA双生病毒的基因沉默。除了基因沉默之外，其他机制也可能控制高温下双生病毒的积累。在实施以PTGS为基础的控制植物病毒积累的策略时，应考虑到这里提出的研究结果。
Short-interfering RNAs ( siRNAs), the molecular markers of posttranscriptional gene silencing (PTGS), are powerful tools that interfere with gene expression and counter virus infection both in plants and animals. Here, we report the effect of temperature on geminivirus-induced gene silencing by quantifying virus-derived siRNAs and by evaluating their distribution along the virus genome for isolates of five species of cassava geminiviruses in cassava ( Manihot esculenta, Crantz) and Nicotiana benthamiana. Cassava geminivirus-induced RNA silencing increased by raising the temperature from 25 degrees C to 30 degrees C, with the appearance of less symptomatic newly developed leaves, irrespective of the nature of the virus. Consequently, nonrecovery-type geminiviruses behaved like recovery-type viruses under high temperature. Next, we evaluated the distribution of virus-derived siRNAs on the respective virus genome at three temperatures ( 25 degrees C, 25 degrees C-30 degrees C, and 30 degrees C). For recovery-type viruses, siRNAs accumulated at moderately higher levels during virus-induced PTGS at higher temperatures, and there was no change in the distribution of the siRNA population along the virus genome. For nonrecovery-type viruses, siRNAs accumulated at strikingly higher levels than those observed for infections with recovery- type viruses at high temperature. As determined for an RNA virus, temperature influences gene silencing for single-stranded DNA geminiviruses. It is possible that other mechanisms besides gene silencing also control geminivirus accumulation at high temperatures. The findings presented here should be taken into consideration when implementing PTGS-based strategies to control plant virus accumulation.