The relationship between protein synthesis and protein degradation in object recognition memory
The relationship between protein synthesis and protein degradation in object recognition memory
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DOI：
10.1016/j.bbr.2015.07.038
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发表时间：
2015-11-01
影响因子：
2.7
通讯作者：
Izquierdo, Ivan
中科院分区：
文献类型：
--
作者：
Furini, Cristiane R. G.;Myskiw, Jociane de C.;Izquierdo, Ivan
几十年来，人们一致认为从头合成蛋白质对长期记忆是必要的。第二轮蛋白质合成已被描述为灭绝和重新巩固后，一个未加强的测试会话。最近的研究表明，蛋白质的巩固和再巩固不仅依赖于蛋白质的合成，还依赖于泛素-蛋白酶体系统（UPS）对蛋白质的降解，这是蛋白质周转的主要机制。然而，UPS在物体识别记忆的巩固和再巩固中的作用尚不清楚。在此，我们研究了背侧海马CA1区ups介导的蛋白质降解在物体识别记忆巩固和再巩固中的作用。在海马背侧CA1区立体定向植入输注管的动物进行物体识别任务。在已知影响其他形式记忆（抑抑性回避，水迷宫中的空间学习）的剂量下，UPS抑制剂β - lactacystin不影响物体识别记忆的巩固和再巩固，而蛋白质合成抑制剂anisomycin则损害物体识别记忆的巩固和再巩固。然而，β - lactacystin能够逆转由大霉素引起的海马CA1区再巩固过程的损伤。因此，有可能假设在物体识别记忆的再巩固过程中蛋白质降解和蛋白质合成之间存在直接联系。(C) 2015 Elsevier B.V.版权所有
For decades there has been a consensus that de novo protein synthesis is necessary for long-term memory. A second round of protein synthesis has been described for both extinction and reconsolidation following an unreinforced test session. Recently, it was shown that consolidation and reconsolidation depend not only on protein synthesis but also on protein degradation by the ubiquitin-proteasome system (UPS), a major mechanism responsible for protein turnover. However, the involvement of UPS on consolidation and reconsolidation of object recognition memory remains unknown. Here we investigate in the CA1 region of the dorsal hippocampus the involvement of UPS-mediated protein degradation in consolidation and reconsolidation of object recognition memory. Animals with infusion cannulae stereotaxically implanted in the CA1 region of the dorsal hippocampus, were exposed to an object recognition task. The UPS inhibitor beta-Lactacystin did not affect the consolidation and the reconsolidation of object recognition memory at doses known to affect other forms of memory (inhibitory avoidance, spatial learning in a water maze) while the protein synthesis inhibitor anisomycin impaired the consolidation and the reconsolidation of the object recognition memory. However,beta-Lactacystin was able to reverse the impairment caused by anisomycin on the reconsolidation process in the CA1 region of the hippocampus. Therefore, it is possible to postulate a direct link between protein degradation and protein synthesis during the reconsolidation of the object recognition memory. (C) 2015 Elsevier B.V. All rights reserved.