A live attenuated combination vaccine evokes effective immune-mediated protection against Edwardsiella tarda and Vibrio anguillarum
A live attenuated combination vaccine evokes effective immune-mediated protection against Edwardsiella tarda and Vibrio anguillarum
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DOI：
10.1016/j.vaccine.2014.08.074
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发表时间：
2014-10-14
期刊：
影响因子：
5.5
通讯作者：
Zhang, Yuanxing
中科院分区：
文献类型：
--
作者：
Gao, Yuan;Wu, Haizhen;Zhang, Yuanxing
迟缓爱德华氏菌（Edwardsiellatarda）和鳗弧菌（Vibrioanguillarum）是引起养殖鱼类爱德华氏菌病和弧菌病的两种主要病原菌。在我们以前的研究中，E. tarda WED和V.anguillarum MVAV 6203在接种的斑马鱼或大菱鲆用强毒E. tarda或鳗弧菌（V.anguillarum）。此外，接种疫苗的鱼通过不同的抗原加工和呈递途径加工两种疫苗。在这里，由E. tarda WED和V.anguillarum MVAV 6203最初在斑马鱼中评估。用E.在接种后1个月，接种的斑马鱼分别表现出70%和90%的相对保护存活率。在免疫斑马鱼的肝脏和脾脏中测量与抗原识别、加工和呈递相关的基因的表达。基因表达谱分析表明，一个以上的Toll样受体信号通路被激活，并引起抗原加工和呈递的MHC I和II途径。随后，在大菱鲆中评价了组合疫苗的免疫保护，并且其显示出类似有效的免疫介导的保护。通过ELISA分析，我们发现，在接种疫苗后2个月内，免疫大菱鲆的特异性抗体水平比单一疫苗免疫的鱼有所增加。同时，免疫大菱鲆的肝、脾和肾中MHC I和II的表达水平均上调，表明免疫大菱鲆与免疫斑马鱼一样，激活了抗原加工和呈递的MHC I和II途径。综上所述，本研究成功地研制了大肠杆菌减毒活疫苗。tarda WED和V.anguillarum MVAV 6203的效果较好，具有推广应用前景。(C)2014爱思唯尔有限公司版权所有。
Edwardsiella tarda and Vibrio anguillarum are the two main pathogenic bacteria that cause edwardsiellosis and vibriosis in various species of fish raised in aquaculture. In our previous study, the live attenuated vaccines E. tarda WED and V. anguillarum MVAV6203 showed robust relative protection when vaccinated zebrafish or turbot were challenged with virulent E. tarda or V. anguillarum, respectively. Additionally, vaccinated fish processed the two vaccines through different pathways of antigen processing and presentation. Here, the immune protection of a combination vaccination consisting of E. tarda WED and V. anguillarum MVAV6203 was initially evaluated in zebrafish. After challenge with E. tarda and V. anguillarum at 1 month post-vaccination, the vaccinated zebrafish exhibited the relative protective survival of 70% and 90%, respectively. The expression of genes related to antigen recognition, processing and presentation were measured in the liver and spleen of vaccinated zebrafish. Gene expression profiling showed that more than one Toll-like receptor signaling pathway was activated and that both MHC I and II pathways of antigen processing and presentation were evoked. Later, the immune protection of the combination vaccine was evaluated in turbot and it showed similarly effective immune-mediated protection. By ELISA analysis, we found that the specific antibody levels in vaccinated turbot increased compared to those of fish vaccinated by a single vaccine during 2 months post-vaccination. Meanwhile, the expression levels of MHC I and II in the liver, spleen and kidney of vaccinated turbot were both up-regulated, suggesting that the MHC I and II pathways of antigen processing and presentation are activated in vaccinated turbot, similar to vaccinated zebrafish. In summary, a combination vaccine of live attenuated E. tarda WED and V. anguillarum MVAV6203 is effective and could be used widely in the future. (C) 2014 Elsevier Ltd. All rights reserved.