(-)-Epigallocatechin-3-gallate prevents photocarcinogenesis in mice through interieukin-12-dependent DNA repair (Retracted article. See vol. 78, pg. 6709, 2018)
(-)-Epigallocatechin-3-gallate prevents photocarcinogenesis in mice through interieukin-12-dependent DNA repair (Retracted article. See vol. 78, pg. 6709, 2018)
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DOI：
10.1158/0008-5472.can-06-0218
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发表时间：
2006-05-15
期刊：
影响因子：
11.2
通讯作者：
Katiyar, Santosh K.
中科院分区：
文献类型：
--
作者：
Meeran, Syed M.;Mantena, Sudheer K.;Katiyar, Santosh K.
We have shown previously that topical application of epigallocatechin-3-gallate (EGCG), the major polyphenol of green tea, prevents photocarcinogenesis in mice. EGCG prevents UVB-induced immunosuppression by inducing interleukin-12 (IL-12). As immunosuppression is a risk factor for photocarcinogenesis, we investigated the possibility that EGCG also prevents UVB-induced photocarcinogenesis through an IL-12-dependent DNA repair mechanism. To investigate this possibility, we determined the effects of EGCG on photocarcinogenesis in IL-12 knockout (KO) mice using the formation of cyclobutane pyrimidine dimers (CPD) as an indicator of the extent of UVB-induced DNA damage. Topical application of EGCG (1 mg/cm(2) skin) prevented photocarcinogenesis in wild-type (C3H/HeN) mice in terms of tumor incidence and tumor multiplicity but did not prevent photocarcinogenesis in IL-12 KO mice. UVB-induced DNA damage, as determined by the formation of CPDs and the number of sunburn cells, was resolved more rapidly in the skin of wild-type mice treated with EGCG than untreated control mice. In contrast, the extent of UVB-induced DNA damage and the numbers of sunburn cells were not significantly different in the EGCG-treated IL-12 KO mice and untreated control mice. In addition, treatment of XPA-proficient human fibroblast cells with EGCG promoted repair of UVB-induced CPDs in a dose-dependent manner but not in an XPA-deficient cells, indicating that the nucleotide excision repair mechanism is involved in EGCG-mediated DNA repair. Taken together, these results indicate for the first time that EGCG can prevent photocarcinogenesis through an EGCG-induced IL-12-dependent DNA repair mechanism.
