MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells
MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells
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DOI：
10.1016/s0006-3495(02)75537-9
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发表时间：
2002-04-01
影响因子：
3.4
通讯作者：
Mooseker, MS
中科院分区：
文献类型：
--
作者：
Tyska, MJ;Mooseker, MS
肾上皮细胞系LLC-PK 1-CL 4（CL 4）在其顶面形成有序的刷状缘（BB）。CL 4细胞用于使用GFP标记的MIA（GFP-M1A）、MIA运动域（GFP-MDIQ）和尾域（GFP-Tail）检查BB内MYO 1A（M1A;以前的BIB肌球蛋白1）的动力学。GFP-β-肌动蛋白（GFP-肌动蛋白）用于评估BB内的肌动蛋白动力学。GFP-M1A、GFP-尾而非GFP-MDIQ定位于BB，表明尾足以用于M1A的顶端靶向。GFP-肌动蛋白靶向细胞的所有肌动蛋白结构域，包括BB。光漂白分析后的荧光恢复显示，BB中的GFP-M1A和GFIP-尾转换是快速的，类似于80%的完成。
The kidney epithelial cell line, LLC-PK1-CL4 (CL4), forms a well ordered brush border (BB) on its apical surface. CL4 cells were used to examine the dynamics of MYO1A (M1A; formerly BIB myosin 1) within the BB using GFP-tagged MIA (GFP-M1A), MIA motor domain (GFP-MDIQ), and tail domain (GFP-Tail). GFP-beta-actin (GFP-Actin) was used to assess actin dynamics within the BB. GFP-M1A, GFP-Tail, but not GFP-MDIQ localized to the BB, indicating that the tail is sufficient for apical targeting of M 1 A. GFP-Actin targeted to all the actin domains of the cell including the BB. Fluorescence recovery after photobleaching analysis revealed that GFP-M1A and GFIP-Tail turnover in the BB is rapid, similar to80% complete in