Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the α subunit of protein kinase CK2
Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the α subunit of protein kinase CK2
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DOI：
10.1073/pnas.111136798
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发表时间：
2001-07-03
影响因子：
11.1
通讯作者：
Yano, M
中科院分区：
文献类型：
--
作者：
Takahashi, Y;Shomura, A;Yano, M
hd 6是一个与水稻光周期敏感性有关的数量性状位点。在粳稻品种日本晴和籼稻品种Kasalath杂交的回交后代中检测到。为了分离Hd 6的基因，我们使用了一个大的分离群体进行Hd 6的高分辨率和精细规模的定位，并在Hd 6区域周围构建了基因组克隆重叠群。用Pi衍生的人工染色体克隆衍生的DNA标记进行的连锁分析将Hd 6限定到26，4-kb的基因组区域。我们确定了一个基因编码的cu亚单位的蛋白激酶CK 2（CK 2 α）在这个地区。CK 2a的日本晴等位基因包含一个提前终止密码子，并且所产生的截短产物无疑是无功能的。遗传互补分析表明，CK 2 α的Kasalath等位基因增加抽穗天数。图位克隆与先进的回交后代使我们能够确定一个基因的数量性状位点，即使它表现出相对较小的影响表型。
Hd6 is a quantitative trait locus involved in rice photoperiod sensitivity. It was detected in backcross progeny derived from a cross between the japonica variety Nipponbare and the indica variety Kasalath. To isolate a gene at Hd6, we used a large segregating population for the high-resolution and fine-scale mapping of Hd6 and constructed genomic clone contigs around the Hd6 region. Linkage analysis with Pi-derived artificial chromosome clone-derived DNA markers delimited Hd6 to a 26,4-kb genomic region. We identified a gene encoding the cu subunit of protein kinase CK2 (CK2 alpha) in this region. The Nipponbare allele of CK2a contains a premature stop codon, and the resulting truncated product is undoubtedly nonfunctional. Genetic complementation analysis revealed that the Kasalath allele of CK2 alpha increases days-to-heading. Map-based cloning with advanced backcross progeny enabled us to identify a gene underlying a quantitative trait locus even though it exhibited a relatively small effect on the phenotype.