Communication between viruses guides lysis-lysogeny decisions.
Communication between viruses guides lysis-lysogeny decisions.
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DOI：
10.1038/nature21049
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发表时间：
2017-01-26
期刊：
影响因子：
64.8
通讯作者：
Sorek R
中科院分区：
文献类型：
--
作者：
Erez Z;Steinberger-Levy I;Shamir M;Doron S;Stokar-Avihail A;Peleg Y;Melamed S;Leavitt A;Savidor A;Albeck S;Amitai G;Sorek R
温带病毒可以在宿主细胞中休眠，这一过程称为溶原性。在每一次感染中，这些病毒需要在裂解和溶原性循环之间做出决定，即，无论是复制和裂解宿主还是溶原化并保持宿主存活。在这里，我们表明，spBeta组的病毒（SIV）使用小分子通信系统来协调裂解-溶原性决策。在其芽孢杆菌宿主细胞的感染期间，噬菌体产生释放到培养基中的6aa通讯肽。在随后的感染中，子代噬菌体测量该肽的浓度，如果浓度足够高，则进行溶原化。我们发现，不同的噬菌体编码不同版本的通信肽，展示了一个噬菌体特异性的肽通信代码溶原性的决定。我们将这种通信系统称为“仲裁”系统，并进一步表明它由3个噬菌体基因编码：aimP，产生肽，aimR，细胞内肽受体，和aimX，溶原性的负调节因子。仲裁系统使后代噬菌体能够与其前辈进行通信，即，以估计最近的先前感染的量，并因此决定是否采用溶解或溶原循环。
Temperate viruses can become dormant in their host cells, a process called lysogeny. In every infection, such viruses need to decide between the lytic and the lysogenic cycles, i.e., whether to replicate and lyse their host or to lysogenize and keep the host viable. Here we show that viruses (phages) of the spBeta group use a small-molecule communication system to coordinate lysis-lysogeny decisions. During infection of its Bacillus host cell, the phage produces a 6aa communication peptide that is released to the medium. In subsequent infections, progeny phages measure the concentration of this peptide and lysogenize if the concentration is sufficiently high. We found that different phages encode different versions of the communication peptide, demonstrating a phage-specific peptide communication code for lysogeny decisions. We termed this communication system the “arbitrium” system, and further show that it is encoded by 3 phage genes: aimP, producing the peptide, aimR, the intracellular peptide receptor, and aimX, a negative regulator of lysogeny. The arbitrium system enables an offspring phage to communicate with its predecessors, i.e., to estimate the amount of recent prior infections and hence decide whether to employ the lytic or lysogenic cycle.