Immunohistochemical localization of N-methyl-D-aspartate and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor subunits in the substantia nigra pars compacta of the rat
Immunohistochemical localization of N-methyl-D-aspartate and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor subunits in the substantia nigra pars compacta of the rat
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DOI：
10.1016/s0306-4522(98)00328-5
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发表时间：
1999-03-01
期刊：
影响因子：
3.3
通讯作者：
Standaert, DG
中科院分区：
文献类型：
--
作者：
Albers, DS;Weiss, SW;Standaert, DG
黑质背侧部的离子型谷氨酸受体调节多巴胺神经元的活动。我们使用双标记免疫荧光和激光共聚焦显微镜研究两种类型的离子型受体的亚基在大鼠黑质丘脑腹侧部的定位。N-甲基-D-天冬氨酸受体1和谷氨酸受体2/3的免疫染色在所有酪氨酸羟化酶免疫阳性细胞的索马和近端树突中突出，而仅存在少量的N-甲基-D-天冬氨酸受体2A和N-甲基-D-天冬氨酸受体2B。选择性抗体用于确定存在的N-甲基-D-天冬氨酸受体1的亚型。N1、C1和C2剪接片段的N-甲基-D-天冬氨酸受体1的免疫反应在黑质丘脑腹侧部是罕见的，而N-甲基-D-天冬氨酸受体1的替代C2'末端的免疫反应是相当丰富的。谷氨酸受体1染色是异质性的，约一半的酪氨酸羟化酶免疫阳性细胞染色强烈，而另一半是免疫阴性。谷氨酸受体1染色的细胞集中在黑质腹侧层。谷氨酸受体4也没有发现酪氨酸羟化酶免疫阳性细胞内的黑质parsaprusta.Together，这些数据表明，多巴胺能神经元在黑质parsaprusta. Together主要表达谷氨酸受体1，谷氨酸受体2/3和，N-甲基-D-天冬氨酸受体1亚型含有替代C2'末端。(C)1998年IBRO。出版社：Elsevier Science Ltd
Ionotropic glutamate receptors in the substantia nigra pars compacta regulate the activity of dopamine neurons. We have used dual-label immunofluoresence and confocal laser microscopy to study the localization of subunits of two types of ionotropic receptors within the substantia nigra pars compacta of the rat. Immunostaining for N-methyl-D-aspartate receptor 1 and glutamate receptor 2/3 was prominent in the soma and proximal dendrites of all tyrosine hydroxylase-immunopositive cells, while only low amounts of N-methyl-D-aspartate receptor 2A and N-methyl-D-aspartate receptor 2B were present. Selective antibodies were used to determine the isoforms of N-methyl-D-aspartate receptor 1 present. Immunostaining for the N1, C1 and C2 variably spliced segments of N-methyl-D-aspartate receptor 1 were scarce in the substantia nigra pars compacta, while immunoreactivity for the alternative C2' terminus of N-methyl-D-aspartate receptor 1 was quite abundant. Staining for glutamate receptor 1 was heterogeneous; about half of the tyrosine hydroxylase immunopositive cells stained intensely, while the other half were immunonegative. The glutamate receptor 1-stained cells were concentrated in the ventral tier of the substantia nigra pars compacta. Glutamate receptor 4 was nor found in tyrosine hydroxylase-immunopositive cells within the substantia nigra pars compacta.Together, these data demonstrate that dopaminergic neurons in the substantia nigra pars compacta express primarily glutamate receptor 1, glutamate receptor 2/3 and,N-methyl-D-aspartate receptor 1 isoforms containing the alternative C2' terminus. (C) 1998 IBRO. Published by Elsevier Science Ltd.